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| Status |
Public on Sep 17, 2021 |
| Title |
Pig backfat miRNA profiling |
| Organism |
Sus scrofa |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
Purpose: Analysis of the effect of different fats and amonut of cDDGS in the feedstuff on miRNA expression in porcine backfat
Methods: miRNA-seq analysis was performed on backfat samples collected from 24 male and female crossbred fatteners originating from sows (Polish Landrace × White Large Polish) mated with a boar (Duroc × Pietrain) divided into four dietary groups: 7-cDDGS+rapeseed oil (group I), n=6 (+cDDGS+rapeseed oil -group II), n= 6 (+cDDGS+beeftallow -group III),n=5 (+cDDGS+coconut oil -group IV). The miRNA libraries were constructed from total RNA using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to the manufacturer protocol. The quantification of the obtained libraries was performed on a Qubit 2.0 spectrophotometer (Invitrogen, Life Technologies), while a quality control on a TapeStation 2200 instrument (D1000 ScreenTape; Agilent). 100 single-end cycle sequencing was performed on the HiScanSQ platform (Illumina) with the use of TruSeq SR Cluster Kit v3- CBOT-HS and TruSeq SBS Kit v 3 - HS (Illumina). MicroRNA differentially expressed between dietary groups were identified with the DESeq2 software.
Results: The comparison of miRNA profiles between dietary groups showed The highest number of miRNAs with altered expression was identified in the comparison of animals fed the diet containing cDDGS and coconut oil (group IV) with animals from the –cDDGS + rapeseed oil (group I) (37 miRNA, p adjusted <0.01). Moreover, in comparison between the group IV and groups III and II , 29 (12 upregulated and 17 downregulated in +cDDGS+coconut oil group) and 28 (10 upregulated and 18 downregulated in +cDDGS+coconut oil group) miRNAs were identified, respectively (p adjusted <0.1)
Conclusions: Obtained results suggest that coconut oil induces changes in miRNA profile of backfat in pigs.
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| Overall design |
MicroRNA next generation sequencing of 24 pig backfat samples collected from castrated males and females was performed using Illumina HiScan SQ.
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| Contributor(s) |
Pawlina-Tyszko K, Oczkowicz M |
| Citation missing |
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| |
| Submission date |
Sep 15, 2021 |
| Last update date |
Sep 19, 2021 |
| Contact name |
Klaudia Pawlina-Tyszko |
| E-mail(s) |
klaudia.pawlina@iz.edu.pl
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| Organization name |
National Research Institute of Animal Production
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| Department |
Deaprtment of Animal Genomics and Molecular Biology
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| Lab |
Laboratory of Genomics
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| Street address |
Krakowska 1
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| City |
Balice near Krakow |
| ZIP/Postal code |
32-083 |
| Country |
Poland |
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| Platforms (1) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA763511 |
| SRA |
SRP337223 |
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