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Series GSE184174 Query DataSets for GSE184174
Status Public on Sep 16, 2021
Title Dose dependent expression of extracellular microRNAs in HCT116 colorectal cancer cells exposed to high dose rate ionizing radiation
Platform organism Homo sapiens
Sample organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary Biomarkers of tumour response to radiotherapy could help in optimising cancer treatment. In this study, we focus on identifying changes in extracellular miRNA as a biomarker of radiation-induced damage to human colorectal cancer cells. HCT116 cells were exposed to increasing doses of X-rays, and extracellular miRNAs were analysed by microarray. The results were correlated with frequencies of micronuclei. Fifty-nine miRNAs with positive correlation and four with negative correlation between dose (up to 6 Gy) and expression of extracellular miRNA were verified. In addition, for samples between 0 and 10 Gy, 12 miRNAs into those 59 miRNAs with positive correlation were verified. Of these, these miRNAs showed significantly positive correlation up to 10 Gy between micronucleus frequency and expression of extracellular miRNA. These results suggest that specific miRNAs could be cell damage markers and could serve as secreted radiotherapy response biomarkers for colorectal cancer; however, the results must be validated in serum samples collected from patients undergoing radiotherapy.
Overall design Radiation induced extracellular micro RNA expression in murine colon cancer cell (HCT116) was measured at 72 hours after exposure to doses of 0, 6 and 10 Gy X-rays. Four independent experiments were performed at each doses using different cell culture.
Contributor(s) Monzen S, Ueno T
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Submission date Sep 15, 2021
Last update date Sep 19, 2021
Contact name Satoru Monzen
Phone +81172395959
Organization name Hirosaki University
Street address Hon-cho 66-1
City Hirosaki
State/province Aomori
ZIP/Postal code 036-8564
Country Japan
Platforms (1)
GPL15159 Agilent-031181 Unrestricted_Human_miRNA_V16.0_Microarray 030840 (Probe Name version)
Samples (16)
GSM5579592 0 Gy - #1
GSM5579593 0 Gy - #2
GSM5579594 0 Gy - #3
BioProject PRJNA763507

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE184174_RAW.tar 49.3 Mb (http)(custom) TAR (of TXT)
GSE184174_processed_data_with_probeIDs_and_miRNA_IDs.xlsx 35.6 Kb (ftp)(http) XLSX
Processed data are available on Series record

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