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| Status |
Public on Dec 01, 2021 |
| Title |
Effects of the sex chromosome complement, XX, XO or XY, on the transcriptome and development of mouse oocytes during follicular growth. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Purpose: To understand how sex chromosome complement, XX, XO and XY, influences the transcriptome in the oocytes of grwoth phase.
Methods: Oocytes of 50 and 60 µm in diameter were isolated from mouse ovaries at 18 dpp and subject to RNA-sequencing.
Results: (1) Many X-linked genes are subject to X chromosome dosage dependent expression. (2) Many genes are expressed from both short and long arms of the Y chromosome. (3) The transcriptome landscape in XY oocytes is closer to XX oocytes than XO oocytes. (4) About 10 genes are differentially expressed in XY oocytes compared to XX or XO oocytes.
Conclusions: The differences in XY oocytes became exacerbated to differ from XX or XO oocytes near the end of growth phase.
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| Overall design |
Transcription of XX,XO and XY mouse oocytes during follicular growth
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| Contributor(s) |
Yamazaki W, Badescu D, Tan SL, Ragoussis J, Taketo T |
| Citation(s) |
34987552 |
| |
| Submission date |
Sep 15, 2021 |
| Last update date |
Jan 11, 2022 |
| Contact name |
Dunarel Badescu |
| E-mail(s) |
dunarel@gmail.com
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| Organization name |
McGill University
|
| Department |
Human Genetics
|
| Street address |
740 Dr Penfield Ave
|
| City |
Montreal |
| ZIP/Postal code |
H3A 0G1 |
| Country |
Canada |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA763388 |
| SRA |
SRP337189 |
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