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Series GSE184087 Query DataSets for GSE184087
Status Public on May 18, 2022
Title Expression profiling of MIR181A1HG interference in hMSC-hTERT20 cells
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We identified MIR181A1HG as a lncRNA that is ubiquitously expressed in non-mineralized tissues, highly expressed in proliferating MSCs and decreases in expression during osteogenesis. We demonstrate that knockdown of MIR181A1HG in hMSC-hTERT20 cells by CRISPRi during osteogenic differentiation causes an increase in alkaline phosphatase (ALP) activity and ALPL gene expression. Increased expression of several extracellular matrix proteins was noted. We identified MIR181A1HG as a nuclear lncRNA that binds to chromatin and where it regulates gene expression, including the transcription factor SOX5. These results reveal that MIR181AHG may act as a negative regulator of osteoblast differentiation, thereby providing a novel mechanism of regulatory control of osteogenesis.
Overall design Expression of the lncRNA MIR181AHG was knocked down by CRISPRi using pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP in TERT immortalized human mesenchymal stromal cells (hMSC-hTERT20). Transcriptome changes were examined during osteogenic differentiation at three time points (Day 0, 7 and 14) after lncRNA interference.
Contributor(s) Coralee T, Jonathan G, Jane L, Gary S
Citation(s) 35546168, 37985890
Submission date Sep 14, 2021
Last update date Dec 07, 2023
Contact name Jonathan AR Gordon
Organization name University of Vermont
Department Biochemistry
Street address 89 Beaumont Ave Given E209
City Burlington
State/province VT
ZIP/Postal code 05405
Country USA
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (18)
GSM5577354 hMSC-hTERT20_control_Day0_R1
GSM5577355 hMSC-hTERT20_control_Day0_R2
GSM5577356 hMSC-hTERT20_control_Day0_R3
BioProject PRJNA763162
SRA SRP337013

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Supplementary file Size Download File type/resource
GSE184087_hMSChTERT20_Control_MIR181A1HGkd_normalized_counts.txt.gz 1.9 Mb (ftp)(http) TXT
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