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| Status |
Public on Mar 01, 2024 |
| Title |
Genomic analyses of H1 cells induced by FOXO1 |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Insufficient understanding on cold adaptation technically restricts donor tissue banking. Here we identify FOXO1 as a conserved transcription factor for cell cold survival, relocating from cytosol to nucleus when temperature drops. FOXO1 small ubiquitin-like modifiers (SUMO) -interacting motif (SIM) antagonizes FOXO1-Importin-7 interaction regardless of temperature. At 37°C, SIM enhances FOXO1 interactions with SUMO E3 ligase RANBP2 and Exportin-1, FOXO1 hence cytoplasmic; at 4°C, FOXO1-Importin-7 interaction increases probably via RANBP2, making FOXO1 nuclear. These features enable fine-tuned FOXO1 regulations on chromatin folding and subsequent gene expression. Using these key factors as readouts, a ‘hibernation solution’ was developed, enabling long-term pancreatic islet cold storage and transplantation to cure diabetes. Henceforth, targeting FOXO1 SIM or the SUMO machinery may aid tissue/organ survival from long-term cold and other stresses.
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| |
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| Overall design |
Examination of FOXO1 binding in H1 cells, including two replicates in H1.
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| |
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| Contributor(s) |
Ou J, Zhang X, Liu Y, Liu W, Yang Y |
| Citation(s) |
38570500 |
| |
| Submission date |
Sep 13, 2021 |
| Last update date |
Apr 17, 2024 |
| Contact name |
Liu Yasong |
| E-mail(s) |
liuys3@mail2.sysu.edu.cn
|
| Organization name |
Third Affiliated Hospital Of Sun Yat-Sen University
|
| Street address |
Tianhe District Tianhe Road, No. 600
|
| City |
Guangzhou |
| ZIP/Postal code |
510000 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE185152 |
The RANBP2/Importin-7-FOXO1 axis provides superior cold protection to aid islet transplantation |
|
| Relations |
| BioProject |
PRJNA762679 |
| SRA |
SRP336793 |
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