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Series GSE183594 Query DataSets for GSE183594
Status Public on Sep 08, 2021
Title Gene expression in human keratinocytes cultivated with serum-free medium and serum containing medium
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The objective of this study was thus to develop ab initio that chemically defined serum-free medium. We used a statistical design of experiment (DOE) approach to screen a large number of candidate factors, for both their potential individual and synergetic effects, as to identify a formulation which was then tested extensively.
In this study, we present the properties of keratinocyte cultivated with serum-Free medium (Surge SFM) and complemented DME-Ham medium (with serum).
Overall design Four human keratinocyte populations at passage 2 cultivated with with serum-free medium or serum containing medium until cells attained 80-95% confluence.
Contributor(s) Cortez Ghio S, Barbier M, Doucet E, Debbah I, Le-Bel G, Cartier A, Jolibois E, Morissette A, Larouche D, Fradette J, Guérin S, Garnier A, Germain L
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Submission date Sep 07, 2021
Last update date Sep 10, 2021
Contact name Gaëtan Le-Bel
Organization name CUO-Recherche
Lab Sylvain Guérin
Street address Local H2-00, Hôpital du Saint-Sacrement,1050 Chemin Sainte-Foy
City Québec
State/province Quebec
ZIP/Postal code G1S 4L8
Country Canada
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (8)
GSM5563199 Cell_Bank_cultured_human_keratinocytes_serum-medium_001_rep1
GSM5563200 Cell_Bank_cultured_human_keratinocytes_serum-free-medium_001_rep1
GSM5563201 Cell_Bank_cultured_human_keratinocytes_serum-medium_002_rep1
BioProject PRJNA761384

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE183594_RAW.tar 103.7 Mb (http)(custom) TAR (of TXT)
GSE183594_processed_data.xlsx 2.9 Mb (ftp)(http) XLSX
Processed data included within Sample table
Processed data are available on Series record

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