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| Status |
Public on May 24, 2022 |
| Title |
Nuclear condensation of CDYL links histone crotonylation and cystogenesis in autosomal dominant polycystic kidney disease [ChIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Background:molecular mechanisms coupling cellular metabolisms with the epigenome in cystic cells remains largely unknown
Methods:To investigate the molecular mechanisms underlying the suppression of cyst growth by CDYL overexpression, we generated an engineered ADPKD cell line by stably expressing CDYL with TY1 tag in WT 9-12 human ADPKD cells (CDYL OE). We profiled genome-wide distribution of CDYL through chromatin immunoprecipitation coupled with sequencing (ChIP-seq) analysis in both parental WT 9-12 cells (control) and CDYL OE cells. Genetic overexpression of CDYL reduces histone Kcr. We examined histone Kcr on CDYL-target genes by performing ChIP-seq analyses using antibody recognizing PanKcr and H3K18cr in control and CDYL OE cells.
Results: Using integrative cistromic and transcriptomic analyses, CDYL-regulated cyst-associated genes are identified, whose downregulation depends on CDYL-mediated suppression of histone Kcr.
Conclusions: Thus, through establishing a metabolic niche via nuclear condensation, CDYL connects metabolic changes to transcriptional response via histone Kcr in ADPKD.
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| Overall design |
ChIP sequencing with different antibodies: PanKcr, H3K18cr, and TY1 Tag in control and CDYL OE WT 9-12 cell lines.
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| Contributor(s) |
Cao X, Dang L, Chen Y |
| Citation(s) |
35918147 |
| |
| Submission date |
Sep 06, 2021 |
| Last update date |
Aug 26, 2022 |
| Contact name |
Xinyi Cao |
| E-mail(s) |
cxyldd@tmu.edu.cn
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| Organization name |
Tianjin Medical University
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| Street address |
Qi Xiangtai Road, HePing District, Tianjin, P.R. China
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| City |
Tianjin |
| State/province |
State... |
| ZIP/Postal code |
300070 |
| Country |
China |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (11)
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| This SubSeries is part of SuperSeries: |
| GSE183512 |
Nuclear condensation of CDYL links histone crotonylation and cystogenesis in autosomal dominant polycystic kidney disease |
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| Relations |
| BioProject |
PRJNA761105 |
| SRA |
SRP335864 |
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