|
| Status |
Public on Feb 23, 2022 |
| Title |
Atmospheric particulate matter aggravates CNS demyelination via TLR-4/NF-κB-mediated microglia pathogenic activities [ChIP-seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Atmospheric Particulate Matter (PM) is one of the leading environmental risk factors for the global burden of disease. Increasing epidemiological studies demonstrated that PM plays a significant role in CNS demyelinating disorders; however, there is no direct testimony of this, and yet the molecular mechanism by which the occurrence remains unclear. Using multiple in vivo and in vitro strategies, in the present study we demonstrate that PM exposure aggravates neuroinflammation, myelin injury, and dysfunction of movement coordination ability via boosting microglial pro-inflammatory activities, in both the pathological demyelination and physiological myelinogenesis animal models. Indeed, pharmacological disturbance combined with RNA-seq and ChIP-seq suggests that TLR-4/NF-κB signaling mediated a core network of genes that control PM-triggered microglia pathogenicity. In summary, our study defines a novel atmospheric environmental mechanism that mediates PM-aggravated microglia pathogenic activities, and establishes a systematic approach for the investigation of the effects of environmental exposure in neurologic disorders.
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| |
|
| Overall design |
ChIP-seq of PBS- or PM-treated microglia
|
| |
|
| Contributor(s) |
Zhang Y, Han B |
| Citation(s) |
35199645 |
| |
| Submission date |
Aug 31, 2021 |
| Last update date |
Mar 09, 2022 |
| Contact name |
Yuan Zhang |
| E-mail(s) |
yuanzhang_bio@126.com
|
| Organization name |
Shaanxi Normal University
|
| Street address |
No. 620, West Chang'an Avenue, Chang'an District
|
| City |
Xi'an |
| ZIP/Postal code |
710119 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (4)
|
|
| This SubSeries is part of SuperSeries: |
| GSE183099 |
Atmospheric particulate matter aggravates CNS demyelination via TLR-4/NF-κB-mediated microglia pathogenic activities |
|
| Relations |
| BioProject |
PRJNA759196 |
| SRA |
SRP334925 |
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