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| Status |
Public on Dec 31, 2021 |
| Title |
ChIP-seq on Zfp961 GFP flox/flox mESCs to verify Zfp961 repression capacity to transcription of ERVKs |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We performed ChIP-seq assay with anti-KAP1 antibodies on Zfp961-GFPflox/flox ESCs.We found KAP1 signal decreased at PBS-Lys sites in Zfp961 KO cells compared to WT, suggesting Zfp961 recruiting KAP1 to ERV-K regions. We performed H3K27ac ChIP on Zfp961 WT or KO mESCs. We found that H3k27ac modification at PBS-Lys sites was elevated upon Zfp961 deletion, indicating Zfp961 serving as a repressive transcription factor.
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| Overall design |
Basically, Zfp961-GFP floxed mESCs cell lines treated with 4-OHT or etOH as control were used for anti-KAP1 ChIP and anti-H3K27ac ChIP.
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| Contributor(s) |
Yang B |
| Citation(s) |
35259018 |
| |
| Submission date |
Aug 30, 2021 |
| Last update date |
Mar 31, 2022 |
| Contact name |
Bo Yang |
| E-mail(s) |
yangbo2099@sina.cn
|
| Phone |
15221512158
|
| Organization name |
The Third Affiliated Hospital of Guangzhou Medical University
|
| Lab |
Lab of Sun
|
| Street address |
Guangzhou, 510150, Guangdong Province, China.
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510150 |
| Country |
China |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA758952 |
| SRA |
SRP334738 |
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