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| Status |
Public on May 16, 2022 |
| Title |
Derivation of functional trophoblast stem cells from human primed pluripotent stem cells [scRNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Trophoblast stem cells (TSC) have recently been derived from human embryos, and early first trimester placenta; however, aside from ethical challenges, the unknown disease potential of these cells limits their scientific utility. We have previously established a BMP4-based two-step protocol for differentiation of primed human pluripotent stem cells (hPSC) into functional trophoblast; however, those trophoblast could not be maintained in a self-renewing TSC-like state. Here, we use the first step from this protocol, followed by a switch to a newly-developed TSC media, to derive bona-fide TSC. We show that these cells resemble placenta- and naïve hPSC-derived TSC, based on their transcriptome, as well as in vitro and in vivo differentiation potential. We conclude that primed hPSC can be used to generate functional TSC, through a simple protocol which can be applied to a widely-available set of existing hPSC, including induced pluripotent stem cells derived from patients with known birth outcomes.
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| Overall design |
We differentiated primed PSC into TE-like cells by treatment with BMP4/IWP2 and collected cells at 12h, 24h and 4 days (96h) of treatment. We also collected primed H9 hESC-derived TSC. Single cell libraries were created using the 10x Genomics technology with a target cell number of 1000/cells per sample, pooled and submitted for sc RNA-seq.
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| Contributor(s) |
Soncin F, Morey R, Bui T, Requena Gilbert D, Cheung VC, Kallol S, Kittle R, Farrah OA, Meads M, Pizzo D, Horii M, Fisch K, Parast MM |
| Citation(s) |
35594858 |
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| Submission date |
Aug 25, 2021 |
| Last update date |
Aug 15, 2022 |
| Contact name |
Robert E Morey |
| E-mail(s) |
robmoreyucsd@gmail.com, remorey@health.ucsd.edu
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| Organization name |
UCSD
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| Department |
Pathology
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| Lab |
Parast
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| Street address |
2880 Torrey Pines Scenic Dr
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (5)
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| This SubSeries is part of SuperSeries: |
| GSE182791 |
Derivation of functional trophoblast stem cells from human primed pluripotent stem cells |
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| Relations |
| BioProject |
PRJNA757786 |
| SRA |
SRP334129 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE182790_RAW.tar |
49.0 Mb |
(http)(custom) |
TAR (of CSV) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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