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Series GSE182780 Query DataSets for GSE182780
Status Public on Aug 28, 2021
Title RNA sequencing of patient derived xenograft sample ALL-811 using prime-seq
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We establish a Cre-ERT2-loxP (causes recombination, estrogen receptor mutant T2, locus of X-over P1) based inducible RNAi- (ribonucleic acid interference) mediated gene silencing system in patient-derived xenograft (PDX) models of acute leukemias in vivo. Mimicking anti-cancer therapy in patients, gene inhibition is initiated in mice harboring orthotopic tumors. In fluorochrome guided, competitive in vivo trials, silencing of DUX4 (double homeobox 4) playes an essential role in patients’ leukemias carrying the recently described DUX4-IGH (immunoglobulin heavy chain) translocation, while the downstream mediator DDIT4L (DNA-damage-inducible transcript 4 like) is identified as therapeutic vulnerability.
Overall design DUX4 KD and control PDX cells were isolated out of the murine bone marrow after induction of the shRNA expression by tamoxifen. the mRNA expression pattern was analyzed.
>>> Raw data not provided as German law does not allow human raw sequence data to be publicly available. <<<
Contributor(s) Irmela J, Michela C, Völse K, Becker M, Bagnoli JW
Citation(s) 34580292
Submission date Aug 25, 2021
Last update date Oct 27, 2021
Contact name Irmela Jeremias
Organization name Helmholtz Zentrum München, German Center for Environmental Health (HMGU), Munich, Germany
Department Research Unit Apoptosis in Hematopoietic Stem Cells
Street address Feodor-Lynenstr. 21
City Munich
ZIP/Postal code 81377
Country Germany
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (6)
BioProject PRJNA757755

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Supplementary file Size Download File type/resource
GSE182780_UMI_counts.txt.gz 201.7 Kb (ftp)(http) TXT
Raw data not provided for this record
Processed data are available on Series record

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