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Series GSE182553 Query DataSets for GSE182553
Status Public on May 21, 2022
Title HDAC1/2 is indispensable for mouse and bovine ZGA via regulating distribution of H3K27ac and H3K4me3 [RNA-seq]
Organisms Bos taurus; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Zygotic genome activation (ZGA) is an impressed biological event following fertilization during early embryo development. Precise reprogramming of epigenetic modifications (e.g. histone H3 lysine 27 acetylation, H3K27ac) have been found contribute to safeguard ZGA in zebrafish and Drosophila, but the dynamic of H3K27ac and the underlying regulation mechanism in mammal embryos are still enigmatic yet. As the main erasers for histone acetylation, histone deacetylase 1 and 2 (HDAC1 and HDAC2) are reported essential for mouse oogenesis and embryo lineage development, while it is unclear if they are functional for ZGA. Here, we observed significant global decrease of H3K27ac during ZGA in both mouse and bovine embryos, and accumulation of HDAC1/2 is responsible for the decline of H3K27ac. Repression of HDAC1/2 by dominant negative mutation led to arrested development at late 2-cell stage in mouse embryos, accompanied by downregulation of genes supposed to be expressed during ZGA. ChIP-seq revealed abnormal distribution of H3K27ac, and DUX participates in H3K27ac aberrant hyper-acetylation in embryos overexpressed mutant HDAC1/2. Moreover, suppression of HDAC1/2 also restrains H3K4me3 removal at gene body regions via impeding expression of KDM5s during mouse ZGA, and compensatory exogenous Kdm5b mRNA can fix the exceeding H3K4me3 and failed ZGA. Intriguingly, function of HDAC1/2 seems to be conservative between mouse and bovine embryos. Together, this study reveals that HDAC1/2 is indispensable for mouse and bovine ZGA via regulating distribution of H3K27ac and H3K4me3.
Overall design PolyA RNA profiles of mouse and bovine embryos.
Web link
Contributor(s) Zhang K, Dang Y
Citation(s) 35575026
Submission date Aug 22, 2021
Last update date Oct 11, 2022
Contact name Kun Zhang
Organization name Zhejiang University
Street address 866 Yuhangtang Rd
City Hangzhou
ZIP/Postal code 310058
Country China
Platforms (2)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
GPL26012 Illumina NovaSeq 6000 (Bos taurus)
Samples (26)
GSM5531280 H2O_late_2cell_rep1
GSM5531281 H2O_late_2cell_rep2
GSM5531282 Hdac1_WT_late_2cell_rep1
This SubSeries is part of SuperSeries:
GSE182555 HDAC1/2 is indispensable for mouse and bovine ZGA via regulating distribution of H3K27ac and H3K4me3
BioProject PRJNA756789
SRA SRP333605

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE182553_fpkm_bovine.txt.gz 361.7 Kb (ftp)(http) TXT
GSE182553_fpkm_mouse.txt.gz 1.8 Mb (ftp)(http) TXT
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Raw data are available in SRA
Processed data are available on Series record

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