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Series GSE182540 Query DataSets for GSE182540
Status Public on Apr 24, 2024
Title Premature aging in growth-plate and periosteum resident skeletal stem cells is responsible for bone loss
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Skeleton system has shown high cell heterogeneousity. Different subpopulation of skeleton stem cells or mesenchymal stem cells have been identified by using varied protein markers. However, the decline of which cell types are responsible for age-related bone loss and what characteristic changes of these cells during aging remain to be determined. In the current study, we constructed a conditional premature aging model by deletion of Zmpste24 (Z24) in different cell types. We found that Prx1Cre; Z24fl/fl mice displayed bone loss while OsxCre; Z24fl/fl mice did not, indicating the populations of Prx1Cre +Osxcre- cells are responsible for age-related bone loss. Using single-cell RNA sequencing, we found two populations exit in Prx1Cre; Ai9+ mice but not in OsxCre; Ai9+ mice. Most strikingly, these two populations were declined in Prx1Cre;Z24fl/fl mice, compared to Prx1Cre;Z24fl/+ mice. The two populations, one is Prx1Cre;CD73+ resident in growth plate, called gpSSCs, responsible for trabecular bone loss. Another is Prx1Cre; Sca1+ resident in periosteum, called pSSCs, responsible cortical bone decrease. More importantly, the naturally aged mice also showed the decrease of these two cell types. By integration of chromatin and transcriptional profiling, we found that these two cell populations displayed increased p53 mediated DNA damage and decreased extracellular matrix (ECM) constituents, supporting the degeneration of two populations during aging. Finally, we found that AgrcCreER Z24fl/fl mice displayed the decrease of trabecular bone, but not cortical bone, consistent with AgrcCreER could only label trabecular bone, but not cortical bone. Overall, our study identified two skeleton stem cells, gpSSCs and pSSCs, which are responsible for the age-related bone loss in trabecular bone and cortical bone, respectively.
Overall design To isolate periosteal cells, femurs and tibias were placed in ice-cold PBS after the overlying skin and muscle were carefully removed. The periosteum was scratched using scalpel and forceps gentelly and then incubated with prewarmed digestion solution (2 mg/ml Collagenase (sigma,c6885);2mg/ml Dispase2 (Roche); 200 ug/ml DNase1;10mg/ml Kolliphor P 188 (sigma,15759); 1x HBSS without Mg2+ & Ca2+)at 37°C for 30 minutes. The dissociated periosteal cells were washed twice by centrifugation at 600g for 5 min with ice-cold PBS, 2% FBS. To isolate growth plate cells, dissected growth plates were minced using a scalpel and incubated with 0.15% collagenase(sigma,c6885) at 37°C for 90 minutes on a shaking incubator. Cells were pelleted and resuspended in ice-cold PBS, 2% FBS.The cells were stained with eFluor 450 anti-CD31 (eBioscience,48-0311-80), PerCP/Cy5.5 anti-CD45 (BioLegend, 103132), APC/Cy7 anti-mouse Ter-119 (BioLegend, 116223), BV421 anti mouse CD73 (Biolegend, 127217) or Parcific blue anti Sca1 (BioLegend, 108120) for 30 min. Then cells were measured by BC Cytoflex X after twice of wash by centrifugation at 600g for 5 min with ice-cold PBS, 2% FBS. The data were then analyzed with the CytExpert software.
Contributor(s) Zou W, Yue R, Yang R, Cao D
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Submission date Aug 21, 2021
Last update date Apr 25, 2024
Contact name Dandan Cao
Phone 17853136996
Organization name Tongji University
Street address 同济大学四平校区
City 上海市
ZIP/Postal code 200092
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (28)
GSM5531085 Osx-Cre_scRNAseq
GSM5531086 Prx1-Cre-scRNAseq
GSM5531087 Prx1-ctrl-scRNAseq
BioProject PRJNA756693
SRA SRP333558

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Supplementary file Size Download File type/resource
GSE182540_RAW.tar 2.3 Gb (http)(custom) TAR (of BW, MTX, TSV, TXT, XLS)
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Raw data are available in SRA
Processed data provided as supplementary file

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