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| Status |
Public on Sep 01, 2021 |
| Title |
Single cell RNA-seq of liver cells from B6J mice fed a chow or a high-fat-and-high-sucrose diet |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Cannabinoid receptor-1 (CB-1) blockage in brain is responsible for weight loss, many of the metabolic benefits associated with CB-1 blockade have been attributed to inhibition of CB-1 signaling in the periphery. We produced mice that lacked CB-1 receptors in hepatocytes or stellate cells to determine if CB-1 signaling contributes to the development of NAFLD or liver fibrosis. Deletion of CB-1 receptors in hepatocytes did not alter the development of NAFLD in mice fed a high sucrose high fat diet (HSD) or high fat diet (HFD). Similarly, deletion of CB-1 deletion specifically in stellate cells also did not prevent the development of NAFLD in mice fed the HFD nor did it protect mice for carbon tetrachloride-induced fibrosis. To determine if a small population of cells in liver express CB-1 at high levels either before and/or after HSD feeding, here we performed single cell RNA-sequencing of livers from wild-type mice fed chow or a HSD for 17 weeks and analyzed CB-1 expression. Single-cell sequencing of hepatocytes and stellate cells reveals low Cnr1 expression in livers of mice fed a chow or HSD. Combined, these studies do not support a direct role for hepatocyte or stellate cell CB-1 signaling in the development of NAFLD or liver fibrosis.
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| Overall design |
Two pairs of mice (one pair fed a chow, one pair fed a high-fat-and-high-sucrose diet for 17 weeks) were used for single cell sequencing. For each pair, one mouse was perfused for hepatocyte preparation, and the other for stellate cells preparation. All cells were collected as single cell suspension.
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| Contributor(s) |
Wang S, Horton JD |
| Citation(s) |
34499619 |
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| Submission date |
Aug 18, 2021 |
| Last update date |
Dec 13, 2021 |
| Contact name |
Simeng Wang |
| Organization name |
UT Southwestern Medical Center
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| Street address |
5323 Harry Hines
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| City |
Dallas |
| ZIP/Postal code |
75560 |
| Country |
USA |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA756014 |
| SRA |
SRP333124 |
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