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Series GSE1822 Query DataSets for GSE1822
Status Public on Oct 07, 2004
Title EWS-FLI1-transfection of HEK293
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Response of HEK293-cells after transfection with EWS-FLI1. HEK293 cells were transfected with the expression vector pIRES2-EGFP containing type I EWS-FLI1 or empty control vector. For transient transfection standard DEAE dextran method was used and RNA was isolated 48h post transfection. For stable transfection cells were transfected using FuGENE 6 (Roche, Mannheim, Germany) and cells were selected with 400 ug/mL G418. DNA-microarray analysis was performed using Affymetrix HG-U133A microarrays.(see Staege et al. Cancer Res. 2004)
Keywords = HEK293
Keywords = EWS-FLI1
Keywords = Ewing family tumors
Keywords: other
Contributor(s) Staege MS, Hutter C, Neumann I, Foja S, Hattenhorst UE, Hansen G, Afar D, Burdach SE
Citation(s) 15548687
Submission date Oct 05, 2004
Last update date Aug 10, 2018
Contact name Christoph Hutter
Phone +49-345-5577265
Fax +49-345-5577275
Organization name Martin-Luther-University Halle-Wittenberg
Department Universit├Ątsklinik u. Poliklinik f├╝r Kinder- u. Jugendmedizin
Lab Prof. Dr. S.E.G. Burdach
Street address Weinbergweg 22
City Halle (Saale)
State/province Sachsen-Anhalt
ZIP/Postal code 06120
Country Germany
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (9)
GSM31805 HEK293-control 1
GSM31806 HEK293-control 2
GSM31807 HEK293 stably EWS-FLI1-transfected, BULK
BioProject PRJNA90505

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