|
| Status |
Public on Dec 09, 2021 |
| Title |
Circadian key component CLOCK/BMAL1 interferes with segmentation clock in mouse embryonic organoids |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In mammals, circadian clocks are strictly suppressed during early embryonic stages as well as pluripotent stem cells, by the lack of CLOCK/BMAL1 mediated circadian feedback loops. During ontogenesis, the innate circadian clocks emerge gradually at a late developmental stage, then, with which the circadian temporal order is invested in each cell level throughout a body. Meanwhile, in the early developmental stage, a segmented body plan is essential for an intact developmental process and somitogenesis is controlled by another cell-autonomous oscillator, the segmentation clock, in the posterior presomitic mesoderm (PSM). In the present study, focusing upon the interaction between circadian key components and the segmentation clock, we investigated the effect of the CLOCK/BMAL1 on the segmentation clock Hes7 oscillation, revealing that the expression of functional CLOCK/BMAL1 severely interferes with the ultradian rhythm of segmentation clock in induced PSM and gastruloids. RNA sequencing analysis showed that the premature expression of CLOCK/BMAL1 affects the Hes7 transcription and its regulatory pathways. These results suggest that the suppression of CLOCK/BMAL1-mediated transcriptional regulation during the somitogenesis may be inevitable for intact mammalian development.
|
| |
|
| Overall design |
Examination of total RNA in CLOCK/BMAL1-overexpressing mouse embryonic stem cells (ESCs) and induced presomitic mesoderm (iPSM) colonies
|
| |
|
| Contributor(s) |
Umemura Y, Koike N, Yagita K |
| Citation(s) |
34930826 |
| |
| Submission date |
Aug 02, 2021 |
| Last update date |
Feb 02, 2022 |
| Contact name |
Kazuhiro Yagita |
| E-mail(s) |
kyagita@koto.kpu-m.ac.jp
|
| Organization name |
Kyoto Prefectural University of Medicine
|
| Street address |
Kawaramachi-Hirokoji, Kamigyo-ku
|
| City |
Kyoto |
| ZIP/Postal code |
602-8566 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (20)
|
| GSM5494385 |
tetO-Clock/Bmal1 ESC #1 with dox |
| GSM5494386 |
tetO-Clock/Bmal1 ESC #7 without dox |
| GSM5494387 |
tetO-Clock/Bmal1 ESC #7 with dox |
| GSM5494388 |
tetO-Clock/Bmal1DN ESC #5 without dox |
| GSM5494389 |
tetO-Clock/Bmal1DN ESC #5 with dox |
| GSM5494390 |
tetO-Clock/Bmal1DN ESC #6 without dox |
| GSM5494391 |
tetO-Clock/Bmal1DN ESC #6 with dox |
| GSM5494392 |
pHes7-luc iPSM without dox |
| GSM5494393 |
pHes7-luc iPSM with dox |
| GSM5494394 |
tetO-Clock/Bmal1 pHes7-luc iPSM #12 without dox |
| GSM5494395 |
tetO-Clock/Bmal1 pHes7-luc iPSM #12 with dox |
| GSM5494396 |
tetO-Clock/Bmal1 pHes7-luc iPSM #20 without dox |
| GSM5494397 |
tetO-Clock/Bmal1 pHes7-luc iPSM #20 with dox |
| GSM5494398 |
tetO-Clock/Bmal1DN pHes7-luc iPSM #7 without dox |
| GSM5494399 |
tetO-Clock/Bmal1DN pHes7-luc iPSM #7 with dox |
| GSM5494400 |
tetO-Clock/Bmal1DN pHes7-luc iPSM #19 without dox |
| GSM5494401 |
tetO-Clock/Bmal1DN pHes7-luc iPSM #19 with dox |
|
| Relations |
| BioProject |
PRJNA751487 |
| SRA |
SRP330838 |
Less...
More...