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| Status |
Public on Aug 10, 2021 |
| Title |
Transcriptomic analysis of short/branched-chain acyl-coenzyme A dehydrogenase knocked out bMECs revealed its regulatory effect on lipid metabolism |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The 21st century is an era of molecular breeding, leading to fundamental dairy market and production system changes. Milk fat is the main economic trait in dairy cattle production. Our previous study revealed that the ACADSB gene has a crucial regulatory role in lipid metabolism, which can alter the contents of TGs, CHOL and fatty acids by regulating some related gene which involve in lipid metabolism. As usual, when we explored the function of interest genes, we often design interference and overexpression vectors to regulate the expression level of related gene, and transiently transfect vectors into cells, however, it is limited by transfection efficiency. therefore, we committed to finding a stable mode of gene expression. Knocking out of the ACADSB gene in bovine mammalian epithelial cells (bMECs), which represents a promising and effective means of further research on the effects of ACADSB in vivo lipid metabolism of dairy cattle. At the same time, the knock-out ACADSB gene's bMECs could also be useful genetic material and tools for future research into gene functions related to lipid and fatty acid metabolism, which also can directly use for further exploring the gene molecular mechanism or related pathway.
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| Overall design |
RNA sequence between three ACADSB knock out bovine mammalian epithelial cells (bMECs) cell line and BMECs
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| Contributor(s) |
Jiang P, Zhao Z |
| Citation(s) |
34557544 |
| |
| Submission date |
Jul 29, 2021 |
| Last update date |
Sep 29, 2021 |
| Contact name |
Ping Jiang |
| E-mail(s) |
Jiangp@gdou.edu.cn
|
| Phone |
15143059097
|
| Organization name |
College of Coastal Agricultural Sciences, Guangdong Ocean University
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| Department |
College of Coastal Agricultural Sciences, Guangdong Ocean University
|
| Street address |
Mazhang district haida street
|
| City |
Zhanjiang |
| State/province |
Guangdong |
| ZIP/Postal code |
524088 |
| Country |
China |
| |
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| Platforms (1) |
| GPL15749 |
Illumina HiSeq 2000 (Bos taurus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA750762 |
| SRA |
SRP330478 |
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