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Series GSE180975 Query DataSets for GSE180975
Status Public on Aug 25, 2021
Title Fate mapping of single NK cells identifies a type 1 innate lymphoid-like lineage that bridges innate and adaptive recognition of viral infection [scRNAseq_spleen_Ly49H+_steady-state]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Upon viral infection, NK cells expressing certain germline-encoded receptors are selected, expanded and maintained in an adaptive-like manner. Currently, these are thought to differentiate along a common pathway. However, by fate mapping of single NK cells upon murine cytomegalovirus (MCMV) infection, we identified two distinct NK cell lineages that contributed to adaptive-like responses. One was equivalent to conventional NK (cNK) cells while the other was transcriptionally similar to type 1 innate lymphoid cells (ILC1s). ILC1-like NK cells showed splenic-residency and strong cytokine production but also recognized and killed MCMV-infected cells, guided by activating receptor Ly49H. Moreover, they induced clustering of conventional type 1 dendritic cells and facilitated antigen-specific T cell priming early during MCMV infection, which depended on Ly49H and the NK cell-intrinsic expression of transcription factor Batf3. Thereby, ILC1-like NK cells bridge innate and adaptive viral recognition and unite critical features of cNK cells and ILC1s.
 
Overall design One mRNA profile of total Ly49H+ NK cells from uninfected spleen.
 
Contributor(s) Flommersfeld S, Böttcher JP, Ersching J, Flossdorf M, Meiser P, Pachmayr LO, Leube J, Hensel I, Jarosch S, Zhang Q, Chaudhry MZ, Andrä I, Schiemann M, Busch DH, Cicin-Sain L, Sun JC, Gasteiger G, Victora GD, Höfer T, Buchholz VR, Grassmann S
Citation(s) 34437840
Submission date Jul 27, 2021
Last update date Sep 02, 2021
Contact name Sophie Flommersfeld
E-mail(s) sophie.flommersfeld@tum.de
Organization name Technical University of Munich
Department Institute for Medical Microbiology, Immunology and Hygiene
Lab Veit Buchholz
Street address Trogerstr. 30
City München
ZIP/Postal code 81675
Country Germany
 
Platforms (1)
GPL21103 Illumina HiSeq 4000 (Mus musculus)
Samples (1)
GSM5481660 Ly49H+ NK cells_spleen_steady state
This SubSeries is part of SuperSeries:
GSE180978 Fate mapping of single NK cells identifies a type 1 innate lymphoid-like lineage that bridges innate and adaptive recognition of viral infection
Relations
BioProject PRJNA750241
SRA SRP330349

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Supplementary file Size Download File type/resource
GSE180975_RAW.tar 23.5 Mb (http)(custom) TAR (of LOOM, MTX, TSV)
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Raw data are available in SRA
Processed data provided as supplementary file

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