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| Status |
Public on Jan 31, 2024 |
| Title |
Analysis of hnRNP K interacting RNAs in myeloid KG-1a cells |
| Organism |
Homo sapiens |
| Experiment type |
Other
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| Summary |
HNRNPK is part of the minimally deleted region (MDR) in AML del(9q). To understand the function of hnRNP K in pathogenesis of this AML subtype, we analyzed the function of hnRNP K as an RNA binding protein in myeloid cells. Co-immunoprecipitated RNAs from hnRNP K and non-related control immunoprecipitation as well as input RNA were subjected to next generation sequencing. This analysis revealed an interaction of hnRNP K with 1076 RNAs, among them many mRNAs encoding transcription factors involved in myeloid differentiation and AML pathogenesis.
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| Overall design |
Cytoplasmic extracts from KG-1a cells were subjected to immunopreciptiation with a hnRNP K specific antibody or a non-related control reaction. Total RNA from cytoplasmic extract was isolated as input. Three independent biological replicates were analyzed.
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| Contributor(s) |
Rahn K, Abdallah AT, Gan L, Sonntag R, Marx G, Brümmendorf TH, Denecke B, Ostareck DH, Ostareck-Lederer A, Crysandt M, Naarmann-de Vries IS |
| Citation missing |
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| |
| Submission date |
Jul 26, 2021 |
| Last update date |
Jan 31, 2024 |
| Contact name |
Ali T Abdallah |
| E-mail(s) |
bioinformatics.ali.abdallah@gmail.com
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| Organization name |
University of Cologne
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| Department |
Medical Faculty
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| Lab |
CECAD
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| Street address |
Joseph Stelzmann Str. 26
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| City |
Cologne |
| ZIP/Postal code |
50931 |
| Country |
Germany |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA749749 |
| SRA |
SRP329905 |
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