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Status |
Public on Dec 08, 2022 |
Title |
ES-derived Dlx6a-Cre fate-mapped neuron bulk RNA-seq |
Organism |
Mus musculus |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Parental ES reporter line Dlx6a-Cre; Ai9 was differentiated using 2 different transcriptional specifiation strategies: 1) Nkx2-1/Dlx2 or 2) Nkx2-1/Dlx2/St18. Neurons were directeed towards neural, telencephalic and then ventral telencephalic identity prior to neuronal differentiation and reporter gene activation. Cells were differentiated for 12 days. tdTomato+ neurons were isolated by flow cytometry prior to RNA isolationa and library construction.
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Overall design |
Comparison of DIFF12 N/D and N/D/S tdTomato+ neurons by bulk RNA-seq
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Contributor(s) |
Au E, Nunnelly L |
Citation(s) |
36517477 |
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Submission date |
Jul 26, 2021 |
Last update date |
Jan 06, 2023 |
Contact name |
Edmund Au |
E-mail(s) |
ea2515@cumc.columbia.edu
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Organization name |
Columbia University Irving Medical Center
|
Street address |
630 West 168th Street, room P&S14-401
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City |
New York |
ZIP/Postal code |
10032 |
Country |
USA |
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Platforms (1) |
GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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Samples (4)
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Relations |
BioProject |
PRJNA749729 |
SRA |
SRP329886 |