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Series GSE180825 Query DataSets for GSE180825
Status Public on Dec 08, 2022
Title ES-derived Dlx6a-Cre fate-mapped neuron bulk RNA-seq
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Parental ES reporter line Dlx6a-Cre; Ai9 was differentiated using 2 different transcriptional specifiation strategies: 1) Nkx2-1/Dlx2 or 2) Nkx2-1/Dlx2/St18. Neurons were directeed towards neural, telencephalic and then ventral telencephalic identity prior to neuronal differentiation and reporter gene activation. Cells were differentiated for 12 days. tdTomato+ neurons were isolated by flow cytometry prior to RNA isolationa and library construction.
 
Overall design Comparison of DIFF12 N/D and N/D/S tdTomato+ neurons by bulk RNA-seq
 
Contributor(s) Au E, Nunnelly L
Citation(s) 36517477
Submission date Jul 26, 2021
Last update date Jan 06, 2023
Contact name Edmund Au
E-mail(s) ea2515@cumc.columbia.edu
Organization name Columbia University Irving Medical Center
Street address 630 West 168th Street, room P&S14-401
City New York
ZIP/Postal code 10032
Country USA
 
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM5471646 neurons, N/D/S replicate #1
GSM5471647 neurons, N/D replicate #1
GSM5471648 neurons, N/D/S replicate #2
Relations
BioProject PRJNA749729
SRA SRP329886

Download family Format
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE180825_RAW.tar 760.0 Kb (http)(custom) TAR (of CSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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