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Series GSE180737 Query DataSets for GSE180737
Status Public on Jul 24, 2021
Title New-Onset IgG Autoantibodies in Hospitalized Patients with COVID-19 [Autoantigen V1]
Platform organisms Escherichia coli; Triticum aestivum; Spodoptera frugiperda; Homo sapiens; Sus scrofa; Bos taurus; Capra aegagrus; Cricetulus griseus
Sample organism Homo sapiens
Experiment type Protein profiling by protein array
Summary We developed three different protein arrays to measure IgG autoantibodies associated with Connective Tissue Diseases (CTDs), Anti-Cytokine Antibodies (ACA), and anti-viral antibody responses in 147 hospitalized COVID-19 patients in three different centers.
 
Overall design Protein arrays were constructed by coupling antigens to carboxylated magnetic beads. Arrays consisting of conjugated beads were then used to probe hospitalized COVID-19 patient serum samples, healthy control samples, and autoimmune prototype samples to identify autoantibodies. Samples were run in duplicate except one sample (UP67 D7) which was run in quadruplicate. for A Luminex FLEXMAP 3D® system was used to quantitatively measure autoantibody reactivity in each sample in units of mean flourescent intensity (MFI). Positive antibody hits were statistically identified by a 5 standard deviation cutoff calculated from the MFI of healthy controls.
 
Contributor(s) Chang SE, Feng A, Meng W, Apostolidis SA, Skevaki C, Luning Prak ET, Utz PJ
Citation(s) 34521836
Submission date Jul 23, 2021
Last update date Oct 13, 2021
Contact name Allan Feng
E-mail(s) afeng1@stanford.edu
Phone 2055678956
Organization name Stanford School of Medicine
Department Imm/Rheum
Lab Utz
Street address 269 Campus Dr
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (1)
GPL30430 Utz Lab autoantigen protein microarray v1
Samples (65)
GSM5469269 autoantigen_V1_Patient KP01
GSM5469270 autoantigen_V1_Patient KP02
GSM5469271 autoantigen_V1_Patient KP03
This SubSeries is part of SuperSeries:
GSE180743 New-Onset IgG Autoantibodies in Hospitalized Patients with COVID-19
Relations
BioProject PRJNA749315

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE180737_Autoantigen_v1_Platform_Raw_MFI.xlsx 371.9 Kb (ftp)(http) XLSX
Processed data included within Sample table

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