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Series GSE180259 Query DataSets for GSE180259
Status Public on Jul 17, 2021
Title RNA-seq Analysis of Wild Type(WT) and Dcaf11 overexpression (OE) ESCs
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Telomeres play vital roles in ensuring chromosome stability and are thus closely linked with the onset of aging and human disease. Telomeres undergo extensive lengthening during early embryogenesis. However, the detailed molecular mechanism of telomere resetting in early embryos remains unknown. Here, we show that Dcaf11 (Ddb1- and Cul4-associated factor 11) participates in telomere elongation in early embryos and 2-cell-like embryonic stem cells (ESCs). The deletion of Dcaf11 in embryos and ESCs leads to reduced telomere sister-chromatid exchange (T-SCE) and impairs telomere lengthening. Importantly, Dcaf11-deficient mice exhibit gradual telomere erosion with successive generations, and hematopoietic stem cell (HSC) activity is also greatly compromised. Mechanistically, Dcaf11 targets Kap1 (KRAB-associated protein 1) for ubiquitination-mediated degradation, leading to the activation of Zscan4 downstream enhancer and the removal of heterochromatic H3K9me3 at telomere/subtelomere regions. Our study therefore demonstrates that Dcaf11 plays important roles in telomere elongation in early embryos and ESCs through activating Zscan4.
Overall design ESCs mRNA profiles of WT and Dcaf11 OE were generated by deep sequencing.
Contributor(s) Le R, Huang Y, Zhang Y
Citation(s) 33357405
Submission date Jul 16, 2021
Last update date Jul 19, 2021
Contact name Yixin Huang
Organization name Tongji University
Street address Siping Road
City Shanghai
ZIP/Postal code 200092
Country China
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (4)
GSM5456755 WT-ES-1
GSM5456756 WT-ES-2
GSM5456757 OE-ES-1
This SubSeries is part of SuperSeries:
GSE132746 Dcaf11 facilitates telomere elongation in early embryos and embryonic stem cells (ESCs) through activating the Zscan4-mediated alternative lengthening telomere (ALT) mechanism
BioProject PRJNA747277
SRA SRP328699

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Supplementary file Size Download File type/resource
GSE180259_RAW.tar 2.2 Mb (http)(custom) TAR (of TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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