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| Status |
Public on Jul 17, 2021 |
| Title |
RNA-seq Analysis of Wild Type(WT) and Dcaf11 overexpression (OE) ESCs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Telomeres play vital roles in ensuring chromosome stability and are thus closely linked with the onset of aging and human disease. Telomeres undergo extensive lengthening during early embryogenesis. However, the detailed molecular mechanism of telomere resetting in early embryos remains unknown. Here, we show that Dcaf11 (Ddb1- and Cul4-associated factor 11) participates in telomere elongation in early embryos and 2-cell-like embryonic stem cells (ESCs). The deletion of Dcaf11 in embryos and ESCs leads to reduced telomere sister-chromatid exchange (T-SCE) and impairs telomere lengthening. Importantly, Dcaf11-deficient mice exhibit gradual telomere erosion with successive generations, and hematopoietic stem cell (HSC) activity is also greatly compromised. Mechanistically, Dcaf11 targets Kap1 (KRAB-associated protein 1) for ubiquitination-mediated degradation, leading to the activation of Zscan4 downstream enhancer and the removal of heterochromatic H3K9me3 at telomere/subtelomere regions. Our study therefore demonstrates that Dcaf11 plays important roles in telomere elongation in early embryos and ESCs through activating Zscan4.
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| Overall design |
ESCs mRNA profiles of WT and Dcaf11 OE were generated by deep sequencing.
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| Contributor(s) |
Le R, Huang Y, Zhang Y |
| Citation(s) |
33357405 |
| |
| Submission date |
Jul 16, 2021 |
| Last update date |
Jul 19, 2021 |
| Contact name |
Yixin Huang |
| E-mail(s) |
1710944@tongji.edu.cn
|
| Organization name |
Tongji University
|
| Street address |
Siping Road
|
| City |
Shanghai |
| ZIP/Postal code |
200092 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE132746 |
Dcaf11 facilitates telomere elongation in early embryos and embryonic stem cells (ESCs) through activating the Zscan4-mediated alternative lengthening telomere (ALT) mechanism |
|
| Relations |
| BioProject |
PRJNA747277 |
| SRA |
SRP328699 |
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