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| Status |
Public on Jan 11, 2022 |
| Title |
Danger signal initiates differentiation of monocytes into osteopontin‑producing macrophages III |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: We report the application of single-cell RNA sequencing for profiling the genexpression of different monocyte types in order to characterize monocytes that differentiate into macrophages under danger signal in comparison to conventional GM-CSF and M-CSF macrophages
Methods: Monocytes freshly isolated from PBMCs were differentiated into GM-CSF, M-CSF, and danger-signal induced macrophages. Two million cells of each macrophage population were tagged accordingly with TotalSeq™‑B Hashtags. Single cell RNA sequencing was performed on all macrophage populations in order to characterize cells from each population. ScRNAseq analysis was performed with the R package Seurat on 16470 barcodes after filtering.
Results: Cluster analysis reveals distinct separation of all three macrophage populations.
Conclusion: The clear difference in overall gene expression profile suggests that macrophages exposed to danger signal differentiate into a novel macrophage type in contrast to conventional GM-CSF and M-CSF monocytes.
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| Overall design |
mRNA profiling of differentiated macrophages
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| Contributor(s) |
Rossol M, Hagemann T |
| Citation(s) |
35022393 |
| |
| Submission date |
Jul 14, 2021 |
| Last update date |
Jan 28, 2022 |
| Contact name |
Manuela Rossol |
| E-mail(s) |
manuela.rossol@medizin.uni-leipzig.de
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| Organization name |
University of Leipzig
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| Lab |
Experimental Rheumatology
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| Street address |
Liebigstr. 19
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| City |
Leipzig |
| ZIP/Postal code |
04103 |
| Country |
Germany |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA746582 |
| SRA |
SRP328345 |
