NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE179943 Query DataSets for GSE179943
Status Public on Dec 15, 2022
Title Single cell gene expression profiling of cell lines and murine peripheral blood mononuclear cells (PBMCs)
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary To demonstrate the efficacy of our developed Automated Live-imaging and cell Picking System for downstream RNA sequencing (ALPS&RNA-seq), we isolated single cells of three cell lines (murine T cell, murine leukemia cell, and murine hematopoietic progenitor cell) and of murine peripheral blood mononuclear cells (PBMCs). The detemined profiles of the three cell lines were different, and the profiles of the PBMSc showed three subpopulations which were determined as CD4+ T cells, CD8+ T cells, and B cells based on the marker genes.
 
Overall design Four 96-well plates containing single cells were isolated from a cell line mixture of murine leukemia cells, T cells, and hematopoietic progenitor cells by ALPS with random selection mode (3mix-random). Four plates of 36 leukemia cells, 36 T cells, 24 hematopoietic progenitor cells in order were isolated by ALPS with target selection mode (3mix-target). Two plates of 36 leukemia cells, 36 T cells, 24 hematopoietic progenitor cells in order were sorted by FACS (3mix-FACS). Sixteen plates of leukemia cells, T cells, and hematopoietic progenitor cells were isolated by ALPS after time-lapse imaging (3mix-timelapse). Eight plates of PBMCs of C57BL/6J mice were isolated by ALPS after time-lapse imaging (PBMC-timelapse).
Web link https://doi.org/10.1073/pnas.2210283120
 
Contributor(s) Jin J, Ogawa T, Shiroguchi K
Citation(s) 36577074
Submission date Jul 12, 2021
Last update date Jan 09, 2023
Contact name Katsuyuki Shiroguchi
E-mail(s) katsuyuki.shiroguchi@riken.jp
Organization name RIKEN
Department Center for Biosystems Dynamics Research (BDR)
Lab Laboratory for Prediction of Cell Systems Dynamics
Street address 6-2-3 Furuedai
City Suita
State/province Osaka
ZIP/Postal code 565-0874
Country Japan
 
Platforms (3)
GPL16417 Illumina MiSeq (Mus musculus)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (3264)
GSM5437509 3mix-random, 3mix-random-plate1_1
GSM5437510 3mix-random, 3mix-random-plate1_2
GSM5437511 3mix-random, 3mix-random-plate1_3
Relations
BioProject PRJNA745953
SRA SRP327980

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE179943_3mix_RNA_expression.txt.gz 9.4 Mb (ftp)(http) TXT
GSE179943_PBMC_RNA_expression.txt.gz 603.0 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap