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Series GSE178867 Query DataSets for GSE178867
Status Public on Jun 25, 2021
Title RNA-seq profiles of rat cardiomyocytes overexpressing hsa-miR-106b-5p, hsa-miR-93-5p, hsa-miR-25-3p or cel-miR-67
Organism Rattus norvegicus
Experiment type Expression profiling by high throughput sequencing
Summary Myocardial regeneration is restricted to early postnatal life, when mammalian cardiomyocytes still retain the ability to proliferate. The molecular cues that induce cell cycle arrest of neonatal cardiomyocytes towards terminally differentiated adult heart muscle cells remain obscure. We report that the miR-106b~25cluster is higher expressed in the early postnatal myocardium and decreases in expression towards adulthood, especially under conditions of overload, and orchestrates the transition of cardiomyocyte hyperplasia towards cell cycle arrest and hypertrophy by virtue of its targetome. To identify the relevant targets of individual miRNAs in the miR-106b~15 cluster and elucidate the molecular mechanisms underlying the proliferative effects of this microRNA cluster, we assessed the global transcriptomic changes by deep-sequencing total neonatal mouse cardiomyocyte RNA after exogeneous transfection with hsa-miR-106b-5p, hsa-miR-93-5p, hsa-miR-25-3p and compared the transcriptomic profiles to cardiomyocytes transfected with cel-miR-67, a control miRNA.
 
Overall design Cardiomyocyte RNA profiles of 1-day old wild type (WT) rats overexpressing individual members of the miR-106b~25 microRNA cluster were generated by deep sequencing using Illumina Hiseq 2000; Four condition experiment; one replicate per condition
 
Contributor(s) de Windt LJ, Dirkx E
Citation(s) 34376683
Submission date Jun 24, 2021
Last update date Sep 08, 2021
Contact name Ryan John A Cubero
E-mail(s) ryanjohn.cubero@ist.ac.at
Organization name IST Austria
Lab Lab Siegert
Street address Am Campus 1
City Klosterneuburg
State/province Lower Austria
ZIP/Postal code 3400
Country Austria
 
Platforms (1)
GPL14844 Illumina HiSeq 2000 (Rattus norvegicus)
Samples (4)
GSM5399471 hsa-miR-106b-5p
GSM5399472 hsa-miR-93-5p
GSM5399473 hsa-miR-25-3p
Relations
BioProject PRJNA741260
SRA SRP325536

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE178867_RAW.tar 1.0 Mb (http)(custom) TAR (of TXT)
GSE178867_RPKM_miRNA_All.txt.gz 650.5 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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