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Status |
Public on Aug 25, 2021 |
Title |
Profiling of microRNA expression of mouse cardiac neural crest cells and non neural crest cells |
Platform organism |
synthetic construct |
Sample organism |
Mus musculus |
Experiment type |
Non-coding RNA profiling by array
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Summary |
In this study, we investigate the miRNA expression profile of cardiac neural crest cells (NCCs) and non-NCCs. Rosa-mT-mG reporter mice, which possess loxP sites on either side of a membrane-targeted tdTomato (mT) cassette and express strong red fluorescence in all tissues and cell types, were applied in this study. The presence of Wnt1-Cre leads to the deletion of the mT cassette and the expression of the membrane-targeted EGFP (mG) cassette located just downstream in NCCs. E10.5 Rosa-mT-mG/Wnt1-Cre EGFP positive embryos were collected. The 3rd, 4th and 6th pharyngeal arches and outflow tract were dissected out, digested and sorted for both EGFP+ cranial NCCs and EGFP- control cells by FACS sorting. Total RNAs were prepared for microRNA microarray from cardiac NCCs and non-NCCs.
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Overall design |
Examine the expression of global microRNAs in cardiac NCCs and non-NCCs
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Contributor(s) |
Chen J, Huang Z |
Citation(s) |
34568467 |
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Submission date |
Jun 24, 2021 |
Last update date |
Sep 29, 2021 |
Contact name |
Youchen Yan |
E-mail(s) |
yanych8@mail2.sysu.edu.cn
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Organization name |
The First Affiliated Hospital, Sun Yat-sen University
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Street address |
No.58 Zhongshan Road II
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City |
Guangzhou |
State/province |
Guangdong |
ZIP/Postal code |
510080 |
Country |
China |
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Platforms (1) |
GPL8786 |
[miRNA-1] Affymetrix Multispecies miRNA-1 Array |
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Samples (2) |
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Relations |
BioProject |
PRJNA741194 |