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Series GSE178823 Query DataSets for GSE178823
Status Public on Aug 25, 2021
Title Profiling of microRNA expression of mouse cardiac neural crest cells and non neural crest cells
Platform organism synthetic construct
Sample organism Mus musculus
Experiment type Non-coding RNA profiling by array
Summary In this study, we investigate the miRNA expression profile of cardiac neural crest cells (NCCs) and non-NCCs. Rosa-mT-mG reporter mice, which possess loxP sites on either side of a membrane-targeted tdTomato (mT) cassette and express strong red fluorescence in all tissues and cell types, were applied in this study. The presence of Wnt1-Cre leads to the deletion of the mT cassette and the expression of the membrane-targeted EGFP (mG) cassette located just downstream in NCCs. E10.5 Rosa-mT-mG/Wnt1-Cre EGFP positive embryos were collected. The 3rd, 4th and 6th pharyngeal arches and outflow tract were dissected out, digested and sorted for both EGFP+ cranial NCCs and EGFP- control cells by FACS sorting. Total RNAs were prepared for microRNA microarray from cardiac NCCs and non-NCCs.
 
Overall design Examine the expression of global microRNAs in cardiac NCCs and non-NCCs
 
Contributor(s) Chen J, Huang Z
Citation(s) 34568467
Submission date Jun 24, 2021
Last update date Sep 29, 2021
Contact name Youchen Yan
E-mail(s) yanych8@mail2.sysu.edu.cn
Organization name The First Affiliated Hospital, Sun Yat-sen University
Street address No.58 Zhongshan Road II
City Guangzhou
State/province Guangdong
ZIP/Postal code 510080
Country China
 
Platforms (1)
GPL8786 [miRNA-1] Affymetrix Multispecies miRNA-1 Array
Samples (2)
GSM5398370 cardiac_neural crest cells_1
GSM5398371 cardiac_non neural crest cells_1
Relations
BioProject PRJNA741194

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE178823_RAW.tar 320.0 Kb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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