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| Status |
Public on Jan 11, 2023 |
| Title |
Deep sequencing data from PFS screen of SuCas12a2 CRISPR-Cas nuclease |
| Organism |
Escherichia coli |
| Experiment type |
Other
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| Summary |
In this work we analyzed PFS sequence recognized by SuCas12a2 Type V CRISPR-Cas nuclease
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| Overall design |
SuCas12a2 was challanged with a library of randomized flanking sequences. Two biological replicates were prepared and analyzed. Each replicate consisted of a library of flankign sequences challanged by SuCas12a2 (pOD4) and a library processed in the absence of SuCas12a2 (pOD49). Sequences were extracted from the Illumina data and their corresponding depletion scores were calculated.
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| Contributor(s) |
Dmytrenko O, Neumann GC, Hallmark T, Keiser DJ, Crowley VM, Vialetto E, Mougiakos I, Wandera KG, Domgaard H, Weber J, Gaudin T, Metcalf J, Gray BN, Begemann MB, Jackson RN, Beisel CL |
| Citation(s) |
36599979 |
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| Submission date |
Jun 21, 2021 |
| Last update date |
Jan 12, 2023 |
| Contact name |
Chase L Beisel |
| Organization name |
Helmholtz Institute for RNA-Based Infection Research
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| Lab |
RSYN
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| Street address |
Josef-Schneider-Str. 2
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| City |
Wurzburg |
| ZIP/Postal code |
97080 |
| Country |
Germany |
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| Platforms (1) |
| GPL16085 |
Illumina MiSeq (Escherichia coli) |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE178536 |
Cas12a2 elicits abortive infection through RNA-triggered destruction of dsDNA |
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| Relations |
| BioProject |
PRJNA739561 |
| SRA |
SRP324794 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE178530_CasO_Depleted_Scores.xlsx |
49.1 Kb |
(ftp)(http) |
XLSX |
| GSE178530_RAW.tar |
80.4 Mb |
(http)(custom) |
TAR (of FASTA, TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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