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Series GSE176526 Query DataSets for GSE176526
Status Public on Feb 15, 2024
Title Linker histone H1 drives heterochromatin condensation via phase separation in Arabidopsis
Organism Arabidopsis thaliana
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
Summary We studied how H1 regulates higher-order chromatin architecture by performing Hi-C. In addition, we tested the effects of H1 domains on nucleosome organisation and DNA methylation by performing MNase-seq and Bisulfite-seq.
Overall design Hi-C was performed with h1 mutant seedlings to examine chromatin interactions in the mutant. To test the functions of the C-terminal intrinsically disordered region (IDR) of H1, we generated H1.1G18IDR which contains the IDR and 18 amino acids of the globular domain nearest the IDR in h1 background. As a control, H1.2 with IDR deleted (H1.2ΔIDR) in h1 background was also generated. These transgenic plants as well as WT and h1 mutant were subject to MNase-seq and Bisulfite-seq to analyse nucleosome repeat length and DNA methylation.
Contributor(s) He S, Yu Y, Wang L, Zhang J, Bai Z, Li G, Li P, Feng X
Citation(s) 38309957
Submission date Jun 10, 2021
Last update date Feb 16, 2024
Contact name Xiaoqi Feng
Organization name John Innes Centre
Department Cell and Developmental Biology
Lab Xiaoqi Feng
Street address Norwich Research Park
City Norwich
State/province Norfolk
ZIP/Postal code NR4 7UH
Country United Kingdom
Platforms (2)
GPL19580 Illumina NextSeq 500 (Arabidopsis thaliana)
GPL23157 HiSeq X Ten (Arabidopsis thaliana)
Samples (14)
GSM5368652 WT_seedling_MNase_rep1
GSM5368653 WT_seedling_MNase_rep2
GSM5368654 h1_seedling_MNase_rep1
BioProject PRJNA736615
SRA SRP323534

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Supplementary file Size Download File type/resource
GSE176526_RAW.tar 1.8 Gb (http)(custom) TAR (of BED, GFF, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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