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Series GSE175661 Query DataSets for GSE175661
Status Public on May 24, 2024
Title Blood DNA methylation altered by Mycobacterium tuberculosis infection in mice
Organism Mus musculus
Experiment type Methylation profiling by high throughput sequencing
Summary Knowing when a person was infected with Mycobacterium tuberculosis (M.tb) is critical as recent infection is the strongest clinical risk factor for progression to TB disease in immunocompetent individuals. However, time since M.tb infection is very difficult to determine in routine clinical practice. We determined whether DNA methylation patterns in the blood correlate with time since M.tb infection or exposure to develop a biomarker for recent exposure. As proof of concept, we found a DNA methylation signature that is present during early infection of mice that persists for at least 5 months post-infection.
Overall design Blood was collected from 54 C57BL/6 mice and 46 BALB/c mice infected with a low dose of M.tb (Erdman), as well as 23 C57BL/6 mice and 28 BALB/c mice that served as uninfected, age-matched controls. Blood was collected at days 7, 14, 30, 62, 90, 120 and 153 post-infection. Whole blood was subjected to reduced representation bisulfite sequencing. We identify stable DNA methylation changes associated with infection.
Contributor(s) Ault RC, Headley CA, Piergallini TJ, Gautam S, Dwivedi V, Carless MA, Turner J
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Submission date May 27, 2021
Last update date May 24, 2024
Contact name Russell Ault
Organization name The Ohio State University College of Medicine
Street address 370 W 9th Ave
City Columbus
State/province OH
ZIP/Postal code 43210
Country USA
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (151)
GSM5343396 Lane1_Sample_1
GSM5343397 Lane1_Sample_2
GSM5343398 Lane1_Sample_3
BioProject PRJNA733167
SRA SRP321639

Download family Format
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Supplementary file Size Download File type/resource
GSE175661_RAW.tar 3.5 Gb (http)(custom) TAR (of BEDGRAPH)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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