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Series GSE175650 Query DataSets for GSE175650
Status Public on Jan 01, 2023
Title Fate restricted stromal fibroblasts and adipocytes demonstrate multi-modal responses to tissue injury
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Adult connective tissues rearrange themselves during injury, disease, and aging. This response is widely considered to involve cross-lineage conversion between two differentiated stromal cell types: fibroblasts and adipocytes. Here, we directly explore the potential plasticity of these cells after injury in the skin. Using single-cell transcriptomics we confirm that adipocyte and fibroblast lineages undergo separate differentiation pathways after injury, avoiding cross-lineage conversion. We performed Single-Cell RNA-seq experiments and employed a fibroblast-lineage specific reporter (En1Cre), labeling a sub-population of fibroblasts termed EPFs (Engrailed-lineage positive fibroblasts). Mature adipocytes were tracked using an adiponectin lineage-specific reporter (AdipoqCre). The two lineage-specific transgenic lines were individually crossed to a double-color fluorescent reporter (R26mTmG), thereby marking mature adipocytes and fibrogenic cells with membrane-bound green. Sorted populations were subjected to SCRB-seq analysis.
Overall design Single-cell transcriptomics on mouse stromal cells after injury in the skin
Contributor(s) Kalgudde Gopal S, Stefanska AM, Ansari M, Schiller HB, Rinkevich Y
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Submission date May 27, 2021
Last update date Jan 03, 2023
Contact name Meshal Ansari
Organization name Helmholtz Zentrum Munich
Street address Ingolst├Ądter Landstrasse 1
City Neuherberg
State/province Bavaria
ZIP/Postal code 85764
Country Germany
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (1)
GSM5343241 mouse skin stromal cells after injury
BioProject PRJNA733149
SRA SRP321616

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Supplementary file Size Download File type/resource
GSE175650_RAW.tar 94.2 Mb (http)(custom) TAR (of H5AD, MTX, TXT)
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Raw data are available in SRA
Processed data provided as supplementary file

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