GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE175614 Query DataSets for GSE175614
Status Public on Jan 25, 2024
Title Global chromatin accessibility using an Assay for Transposase-Accessible Chromatin sequencing (ATAC-seq) reveals epigenomic reprogramming of Adipose tissue macrophages toward proinflammatory and proangiogenic phenotypes in formerly obese mice.
Organism Mus musculus
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary We report sustained changes in the chromatin accessibility landscape of adipose tissue macrophages (ATMs) from high fat diet (HFD)-fed mice persist long after return to regular diet (RD). We compared the data of ATAC-seq performed on nuclei extracted from FACS-sorted ATMs isolated from HFD, RD-RD and HFD-RD-fed mice. Inter-group comparisons revealed the highest diversity and hence the greatest number of differentially accessible regions (DARs) to occur between ATMs from RD-RD and HFD-RD-fed mice, and considerably fewer DARs were identified between ATMs from HFD-RD vs HFD-fed mice. Association of DARs with the nearest gene and gene ontology (GO) enrichment analysis revealed considerable pathway enrichment, especially pathways coding for angiogenesis and inflammatory response, in HFD-RD group when compared to RD-RD group. The results altogether indicated that most changes in chromatin landscape induced by HFD-feeding are maintained as open chromatin positions for a long time, suggesting that HFD-feeding leads to long-term reprograming of ATMs and renders them prone to pro-angiogenic and pro-inflammatory responses.
Overall design We performed ATAC-seq on nuclei extracted from FACS-sorted ATMs isolated from HFD, RD-RD and HFD-RD-fed mice.
Contributor(s) Hata M
Citation(s) 36603072
Submission date May 27, 2021
Last update date Apr 25, 2024
Contact name Mike Sapieha
Organization name Hôpital Maisonneuve-Rosemont
Street address 5415 Boul. L'Assomption
City Montreal
State/province Quebec
ZIP/Postal code H1T2M4
Country Canada
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (7)
GSM5342484 HFD-RD-AT-Macro1
GSM5342485 HFD-RD-AT-Macro2
GSM5342486 RD-RD-AT-Macro1
BioProject PRJNA733072
SRA SRP321570

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE175614_venn_all_macro.xlsx 1.6 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap