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| Status |
Public on Nov 18, 2021 |
| Title |
Protection against autoimmunity is driven by thymic epithelial cell-mediated regulation of Treg development |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
Thymic education of Tregs is superior to negative selection in the prevention of autoimmunity. To address this hypothesis, TCR repertoirs of thymic and peripheral Tcells (including Tregs) from conditional NIK KO and WT mice have been sequenced and compared.
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| Overall design |
conventional CD8, CD4 T cells and Tregs cells have been sorted from thymus, spleen and lung of 3 WT and 3 Foxn1CrexNIKflfl mice. The analysis is based on the sequecing of the respective TCRb RNAs
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| Contributor(s) |
Haftmann C, Ingelfinger F, Zwicky P, Mair F, Flöss S, Riedel R, Durek P, Spalinger M, Friebel E, Leung B, Kieslow J, Waisman A, Mashreghi M, Hühn J, Becher B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
May 20, 2021 |
| Last update date |
Nov 21, 2021 |
| Contact name |
Pawel Durek |
| E-mail(s) |
pawel.durek@drfz.de
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| Organization name |
Deutsches Rheuma-Forschungszentrum
|
| Street address |
Charitéplatz 1
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| City |
Berlin |
| ZIP/Postal code |
10117 |
| Country |
Germany |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (42)
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| Relations |
| BioProject |
PRJNA731351 |
| SRA |
SRP320617 |
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