GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE174641 Query DataSets for GSE174641
Status Public on Oct 12, 2021
Title The histone chaperone FACT facilitates heterochromatin spreading through regulation of histone turnover and H3K9 methylation states
Organism Schizosaccharomyces pombe
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Heterochromatin formation requires several steps involving: nucleation followed by spreading of large, silent domains and its faithful re-establishment and maintenance after each replication cycle. The essential histone chaperone FACT has been implicated in heterochromatin silencing, however, little is known about the specificity of FACT in this process. Here, by applying single cell assays, ChIP and genetics, we show that FACT mutants have defects in the spreading of heterochromatin at subtelomeres and mating type locus in fission yeast. Further, we demonstrate that the transition of H3K9me2 to me3 is impaired in the FACT mutant and we link the spreading function of FACT to its role in histone turnover suppression. We also identify Epe1 as a specific factor that counteracts heterochromatin spreading defects in the FACT mutants. Together, our studies identify FACT as a first histone chaperone that promotes heterochromatin spreading and add to the current models on histone turnover in propagation of epigenetic marks.
Overall design ChIP-seq experiments in wild type and pob3∆ mutant fission yeast.
Contributor(s) Murawska M, Schauer T, Ladurner AG
Citation(s) 34731638
Submission date May 18, 2021
Last update date Dec 09, 2021
Contact name Tamas Schauer
Organization name Helmholtz Zentrum München
Department Institute of Epigenetics and Stem Cells
Street address Feodor-Lynen-Straße 21
City Munich
ZIP/Postal code 81377
Country Germany
Platforms (1)
GPL13988 Illumina HiSeq 2000 (Schizosaccharomyces pombe)
Samples (8)
GSM5321290 H3K9me2_pob3_delta_1
GSM5321291 H3K9me2_pob3_delta_2
GSM5321292 H3K9me2_wildtype_1
BioProject PRJNA730872
SRA SRP320363

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE174641_RAW.tar 1.7 Mb (http)(custom) TAR (of BW)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap