GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE174629 Query DataSets for GSE174629
Status Public on Aug 30, 2022
Title Lymphatic migration of unconventional T cells promotes site-specific immunity in distinct lymph nodes
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The adaptive limb of the immune system consists of antibody producing B cells and CD4 T helper and CD8 cytotoxic T cells. Besides these classical lymphocyte subsets, unconventional T cells exist that are characterized by a more limited repertoire of their TCR chains. Gamma delta (gd), mucosal-associated invariant (MAIT) and natural killer T cells (NKT) are the major cell types comprising this invariant T cell compartment. These cells are found throughout the body in the non- and lymphoid tissues and they recognize antigens that are linked to non-polymorphic antigen-presenting molecules like CD1 and MR1. Functionally, these cells typically recognize lipids, small-molecule metabolites and phosphoantigens that may be pathogen-derived or expressed by tissues in the context of activation or stress responses. Investigating these cell types on functional level as a group, we found that tissue-derived unconventional T cells constantly migrate like dendritic cells to draining lymph nodes. scRNAseq revealed transcriptional homogeneity of these subsets and shared functional outputs that as group, rather than separate entities, are critical to control bacterial infections. Importantly, since every tissue harbors a unique set of invariant T cells with specific differentiation states (Th1-like, Th2-like and Th17-like) every draining lymph node is as well populated by a unique composition of such cells. By comparing different lymph nodes using scRNA in an unbiased manner, we could resolve internodal differences and further demonstrate the functional consequences on humoral and cellular immune responses. The discovery that every lymph node mounts a unique immune response has a direct impact on vaccination strategies and immunotherapy approaches that aims at harnessing invariant T cells.
Overall design Skin draining lymph nodes from a CXCR6-GFP mouse were isolated and pooled. Single cell suspension was created and incubated with antibody mix for sorting. CXCR6-GFP high and CD44 positive cells were sorted and further processed for sequencing.
Contributor(s) Knöpper K, Saliba AE, Kastenmüller W
Citation(s) 36002023
Submission date May 18, 2021
Last update date Sep 01, 2022
Contact name Antoine-Emmanuel Saliba
Phone +49-931-31-81341
Organization name Helmholtz Institute for RNA-based Infection Research
Street address Josef-Schneider-Straße 2 / D15
City Würzburg
ZIP/Postal code 97080
Country Germany
Platforms (1)
GPL24247 Illumina NovaSeq 6000 (Mus musculus)
Samples (6)
GSM5320758 CXCR6, CD44 LN cells (D7)
GSM5320759 CXCR6, Itgb7 T cells in LN 4h pi (A2_2)
GSM5320760 Cirulating (E10_2)
BioProject PRJNA730825
SRA SRP320345

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE174629_RAW.tar 1.1 Gb (http)(custom) TAR (of H5, TAR)
GSE174629_README.txt 327 b (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap