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Series GSE17448 Query DataSets for GSE17448
Status Public on Feb 04, 2010
Title Methylation Pattern of Human Mesenchymal Stromal Cells
Organism Homo sapiens
Experiment type Methylation profiling by array
Summary In this study, we have analyzed DNA methylation changes upon long-term culture and aging of MSC by using the HumanMethylation27 BeadChip assessing 27,578 unique CpG sites. Cells were taken from bone marrow aspirates from iliac crest (BM) of healthy donors or from the caput femoris (HIP) of elderly patients that received femoral head prosthesis.Overall, the methylation pattern was maintained throughout both long-term culture and aging but highly significant differences were observed at specific CpG sites. Notably, methylation changes in MSC were related in long-term culture and aging in vivo.
 
Overall design Mesenchymal stromal cells (MSC) were isolated from human bone marrow as described before (Wagner et al., 2006, Exp Hematol, 34, 536-548; Wagner et al.,2008, PLoS One 21;3(5):e2213.; Wagner et al., PLoS One. 2009 Jun 9;4(6):e5846.)
Cells were always replated when grown to confluency. A sample for DNA isolation was taken at every passage until the cells finally became senescent. For methylation profiles upon long-term culture we used the samples of early passage (P2) and senescent passage (PX). For methylation profiles upon aging we have only used the samples of passage 2 of younger and elderly donors.

Gene expression data of these samples have been previously deposited unter GSE9593 and GSE12274.
 
Contributor(s) Bork S, Pfister S, Witt H, Horn P, Korn B, Ho A, Wagner W
Citation(s) 19895632, 25241740
Submission date Jul 31, 2009
Last update date Jan 02, 2015
Contact name Simone Bork
Organization name University Hospital of Heidelberg
Department Department of Medicine V
Lab Stem Cell Research
Street address INF 410
City Heidelberg
ZIP/Postal code 69120
Country Germany
 
Platforms (1)
GPL8490 Illumina HumanMethylation27 BeadChip (HumanMethylation27_270596_v.1.2)
Samples (16)
GSM435111 MSC-BM_donor275-50yrs_P2
GSM435112 MSC-BM_donor275-50yrs_P10
GSM435113 MSC-BM_donor277-21yrs_P2
Relations
BioProject PRJNA118875

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE17448_RAW.tar 14.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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