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Series GSE173769 Query DataSets for GSE173769
Status Public on Jul 23, 2021
Title Single-cell atlas reveals meningeal leukocyte heterogeneity in the developing mouse brain
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The meninges are important for brain development and pathology. Using single-cell RNA-sequencing, we have generated the first comprehensive transcriptional atlas of neonatal mouse meningeal leukocytes under normal conditions and after perinatal brain injury. We identified almost all known leukocyte subtypes and found differences between neonatal and adult border-associated macrophages, thus highlighting that neonatal border-associated macrophages are functionally immature with regards to immune responses compared to their adult counterparts. We also identified novel meningeal microglia-like populations that appear to participate in white matter development. Early after hypoxic-ischemic insult, neutrophil numbers increased and they exhibited increased granulopoiesis, suggesting that the meninges are an important site of immune cell expansion with implications for the initiation of inflammatory cascades after neonatal brain injury. Our study provides a single-cell-resolution view of the importance of meningeal leukocytes at different stages of development in health and disease.

Overall design Four total meningeal samples, divided in two groups: hypoxic-ischemic (HI) and naïve mice (controls). The samples were processed in two separate runs, each with one HI sample and one naïve sample. All samples were analyzed in terms of gene expression (GEX) and immune profiling (IP) for TCR and BCR clonality,
Contributor(s) Zelco A, Börjesson V, de Kanter J, Lebrero-Fernandez C, Lauschke VM, Rocha-Ferreira E, Nilsson G, Nair S, Svedin P, Bemark M, Hagberg H, Mallard C, Holstege FP, Wang X
Citation(s) 34301765
Submission date May 03, 2021
Last update date Aug 24, 2021
Contact name Xiaoyang Wang
Phone +46 31-786 32 60
Organization name University of Gothenburg
Department Physiology
Street address Medicinaregatan 11
City Gothenburg
ZIP/Postal code 41390
Country Sweden
Platforms (2)
GPL16417 Illumina MiSeq (Mus musculus)
GPL19057 Illumina NextSeq 500 (Mus musculus)
Samples (12)
GSM5277963 GEX Naïve 1
GSM5277964 GEX HI 1
GSM5277965 GEX Naïve 2
BioProject PRJNA726986
SRA SRP318239

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Supplementary file Size Download File type/resource
GSE173769_RAW.tar 199.8 Mb (http)(custom) TAR (of CSV, MTX, TSV)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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