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Series GSE172267 Query DataSets for GSE172267
Status Public on May 20, 2021
Title Neural Cell Adhesion Molecule (NCAM-1) is Required for Ventricular Conduction System Development
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary Cardiac Purkinje cells (PCs) comprise the most distal portion of the ventricular conduction system (VCS) and are essential for synchronous activation of the ventricular myocardium. Contactin-2 (CNTN2), a member of the immunoglobulin superfamily cell adhesion molecules (IgSF-CAMs), was previously identified as a marker of the VCS. Through differential transcriptional profiling we discovered two additional highly enriched IgSF-CAMs in the VCS, NCAM-1 and ALCAM. Immunofluorescence staining showed dynamic expression patterns for each IgSF-CAM during embryonic and early post-natal stages, but ultimately all three proteins became highly enriched in mature PCs. Mice deficient in NCAM-1, but not CNTN2 or ALCAM, exhibited defects in Purkinje cell gene expression and VCS patterning, as well as cardiac conduction disease. Moreover, using ST8sia2 and ST8sia4 knockout mice, we show that inhibition of post-translational modification of NCAM-1 by polysialic acid (PSA) disrupts trafficking of sarcolemmal intercalated disc proteins to Purkinje cell junctional membranes and abnormal expansion of the extracellular space between apposing Purkinje cells. Taken together, our data provide novel insights into the complex developmental biology of the ventricular conduction system.
 
Overall design Extracted RNA from Purkinje cells and Ventricular myocytes of male NCAM-1 WT and KO adult mice in triplicate. WT samples used as control
 
Contributor(s) Delgado C, Khodadadi-Jamayran A, Fishman G
Citation(s) 34100064
Submission date Apr 17, 2021
Last update date Jul 14, 2021
Contact name Alireza Khodadadi-Jamayran
Organization name New York University, NYU Langone Medical Center
Department Division of Advanced Research Technologies (DART)
Lab Applied Bioinformatics Laboratories (ABL)
Street address 550 1st Ave, MSB 304
City New York City
State/province NY
ZIP/Postal code 10016
Country USA
 
Platforms (1)
GPL17021 Illumina HiSeq 2500 (Mus musculus)
Samples (12)
GSM5251144 NCAM-1 KO PC1
GSM5251145 NCAM-1 KO PC2
GSM5251146 NCAM-1 KO PC3
Relations
BioProject PRJNA722661
SRA SRP315185

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Supplementary file Size Download File type/resource
GSE172267_RAW.tar 511.5 Mb (http)(custom) TAR (of BW, TXT)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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