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| Status |
Public on Apr 15, 2024 |
| Title |
RNA sequencing for the gene expression profile changes of shPdgf-d and shControl mESCs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, by RNA sequencing, we found that Pdgf-d knockdown significantly downregulated differentiation associated genes, particularly those of vascular development and angiogenesis. MAPK/ERK signaling related genes level were repressed as well. Collectively, our work reveals that PDGF-D is a novel player in ESCs regulation and suggests potential application for PDGF-D in ESCs culture and vascular lineage derivation.
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| |
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| Overall design |
Examination of differentially expressed genes in shPdgf-d and shControl mESCs, which were performed sequencing in triplicate.
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| |
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| Contributor(s) |
Li X, Lu W, Xu P |
| Citation(s) |
35859222 |
| |
| Submission date |
Apr 15, 2021 |
| Last update date |
Aug 12, 2024 |
| Contact name |
Weisi Lu |
| E-mail(s) |
luweisi3@mail.sysu.edu.cn
|
| Organization name |
Zhongshan Ophthalmic Center, Sun Yat-sen University
|
| Department |
State Key Laboratory of Ophthalmology
|
| Lab |
Xuri Li Lab
|
| Street address |
No.7 Jinsui Road
|
| City |
Guangzhou |
| ZIP/Postal code |
510060 |
| Country |
China |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA722153 |
| SRA |
SRP314936 |
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