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Series GSE171660 Query DataSets for GSE171660
Status Public on Feb 01, 2022
Title Novel Peripheral Blood Allergen Stimulation Test for Non-IgE-mediated Gastrointestinal Food Allergies in Neonates and Infants.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary The lymphocyte stimulation test (LST) helps with the diagnosis of non-IgE-mediated gastrointestinal food allergies. However, The LST requires large volumes of fresh peripheral blood and long culture times.The purpose of this study was to develop a novel LST. we obtained whole blood from patients with non-IgE-GI-FAs to cow’s milk and disease control children. And, we stimulated it with α-casein and Pmix, which is a mixture of four milk protein components (α-casein, κ-casein, α-lactalbumin, or β-lactoglobulin for 24 hours. Then we analyzed genome-wide gene expression using microarray and selected up- or down-regulated genes
Nine putative marker genes, including those known to be involved in allergic inflammation, such as IL2RA, CCL17 and CISH were specific to patient group after stimulation with α-casein. On the other hand, 232 marker genes were specific to patient group after stimulation with Pmix.
Overall design Whole blood samples from non-IgE-mediated gastrointestinal food allergie patients and disease control children were stimulated with α-casein and Pmix for 24 hours and subjected to genome-wide gene expression profiling analysis using microarray chips.
Contributor(s) Yagi H, Takizawa T
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Submission date Apr 07, 2021
Last update date Feb 02, 2022
Contact name Yag Hisako
Organization name Department of Pediatrics, Gunma University Graduate School of Medicine
Street address 3-39-22 Showa-machi
City Maebashi
ZIP/Postal code 371-8511
Country Japan
Platforms (1)
GPL13607 Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version)
Samples (20)
GSM5229882 Patient 1, WB non stimulation
GSM5229883 Patient 1, WB Pmix stimulation
GSM5229884 Patient 2, WB non stimulation
BioProject PRJNA720426

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE171660_RAW.tar 247.3 Mb (http)(custom) TAR (of TXT)
GSE171660_normalized.txt.gz 3.9 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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