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| Status |
Public on Feb 01, 2022 |
| Title |
Novel Peripheral Blood Allergen Stimulation Test for Non-IgE-mediated Gastrointestinal Food Allergies in Neonates and Infants. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The lymphocyte stimulation test (LST) helps with the diagnosis of non-IgE-mediated gastrointestinal food allergies. However, The LST requires large volumes of fresh peripheral blood and long culture times.The purpose of this study was to develop a novel LST. we obtained whole blood from patients with non-IgE-GI-FAs to cow’s milk and disease control children. And, we stimulated it with α-casein and Pmix, which is a mixture of four milk protein components (α-casein, κ-casein, α-lactalbumin, or β-lactoglobulin for 24 hours. Then we analyzed genome-wide gene expression using microarray and selected up- or down-regulated genes
Nine putative marker genes, including those known to be involved in allergic inflammation, such as IL2RA, CCL17 and CISH were specific to patient group after stimulation with α-casein. On the other hand, 232 marker genes were specific to patient group after stimulation with Pmix.
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| Overall design |
Whole blood samples from non-IgE-mediated gastrointestinal food allergie patients and disease control children were stimulated with α-casein and Pmix for 24 hours and subjected to genome-wide gene expression profiling analysis using microarray chips.
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| Contributor(s) |
Yagi H, Takizawa T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 07, 2021 |
| Last update date |
Feb 02, 2022 |
| Contact name |
Yag Hisako |
| E-mail(s) |
hisakoy2004@yahoo.co.jp
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| Organization name |
Department of Pediatrics, Gunma University Graduate School of Medicine
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| Street address |
3-39-22 Showa-machi
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| City |
Maebashi |
| ZIP/Postal code |
371-8511 |
| Country |
Japan |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (20)
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| GSM5229885 |
Patient 2, WB Pmix stimulation |
| GSM5229886 |
Patient 2, WB α-casein stimulation |
| GSM5229887 |
Patient 3, WB non stimulation |
| GSM5229888 |
Patient 3, WB Pmix stimulation |
| GSM5229889 |
Patient 3, WB α-casein stimulation |
| GSM5229890 |
Patient 4, WB non stimulation |
| GSM5229891 |
Patient 4, WB Pmix stimulation |
| GSM5229892 |
Patient 4, WB α-casein stimulation |
| GSM5229893 |
Control 1, WB non stimulation |
| GSM5229894 |
Control 1, WB Pmix stimulation |
| GSM5229895 |
Control 1, WB α-casein stimulation |
| GSM5229896 |
Control 2, WB non stimulation |
| GSM5229897 |
Control 2, WB Pmix stimulation |
| GSM5229898 |
Control 2, WB α-casein stimulation |
| GSM5229899 |
Control 3, WB non stimulation |
| GSM5229900 |
Control 3, WB Pmix stimulation |
| GSM5229901 |
Control 3, WB α-casein stimulation |
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| Relations |
| BioProject |
PRJNA720426 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE171660_RAW.tar |
247.3 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE171660_normalized.txt.gz |
3.9 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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