GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE171590 Query DataSets for GSE171590
Status Public on Apr 07, 2021
Title Profiling astrocyte transcriptional signatures in response to neurons and neuroactive compounds [RNA-seq]
Organisms Homo sapiens; Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The goal of the study is to profile molecular signature of astrocytes in response to neuronal contact and neuroactive chemicals. We performed RNA-seq on (1) mouse embryonic stem cell derived astrocytes (mES_astrocytes) from different time points of culture to determine transcriptomic changes as astrocytes mature over time in culture, (2) mES_astrocytes cocultured with human neurons to determine how neurons affect astrocyte gene expression and (3) mES_astrocytes cultured in presence of neuroactive chemicals (glutamate, gamma-aminobutyric acid, acetylcholine, noradrenaline, and serotonin) to determine transcriptomic changes induced by different types of neuronal signaling.
Overall design mES_astrocytes were collected (1) in duplicate on day 7, day 14, day 21, and day 35 in culture, (2) in triplicate at selective timepoints day 21 and day 35, (3) in duplicate after co-culture with neurons at day 35, and (4) in triplicate after induction with each neuroactive chemical at day 35 for profiling using RNA-seq.
Contributor(s) Sardar D, Lozzi B, Deneen B
Citation(s) 33921461
Submission date Apr 06, 2021
Last update date Jul 07, 2021
Contact name Brittney Lozzi
Organization name Baylor College of Medicine
Street address 1 Baylor Plaza
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
Platforms (2)
GPL19057 Illumina NextSeq 500 (Mus musculus)
GPL19415 Illumina NextSeq 500 (Homo sapiens; Mus musculus)
Samples (35)
GSM5229000 DS1_day7-1 [RNA-seq]
GSM5229001 DS2_day7-2 [RNA-seq]
GSM5229002 DS3_day14-1 [RNA-seq]
This SubSeries is part of SuperSeries:
GSE171595 Mapping Astrocyte Transcriptional Signatures in Response to Neuroactive Compounds
BioProject PRJNA720140
SRA SRP313703

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE171590_coculture_FPKM.csv.gz 403.4 Kb (ftp)(http) CSV
GSE171590_d7-d35_mES_astrocyte_FPKM.csv.gz 704.1 Kb (ftp)(http) CSV
GSE171590_mESastro_d35vsd21_CPM.csv.gz 625.5 Kb (ftp)(http) CSV
GSE171590_mESastrocyte_neurochemical_CPM.csv.gz 1.0 Mb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap