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Series GSE171437 Query DataSets for GSE171437
Status Public on Aug 22, 2023
Title Locational memory of macrovessel vascular cells is transcriptionally imprinted
Organism Canis lupus familiaris
Experiment type Expression profiling by high throughput sequencing
Summary The locational predisposition of vascular pathologies illustrates the need for a better insight into vascular heterogeneity. To investigate the transcriptomic basis of angiodiversity, we isolated and analyzed transcriptomes from endothelial cells and vascular smooth muscle cells from nine different adult canine macrovessels: the aorta, coronary artery, vena cava, portal vein, femoral artery, femoral vein, saphenous vein, pulmonary vein, and pulmonary artery. We identified both reported and novel expression patterns defining specialized adult blood vessels. Our findings also show that adult vascular cells in culture express a remarkably high number of transcription factors crucial to organ development in the embryo. The persistent expression of these genes in culture indicates that these genes are not regulated by the flow or surrounding cell types but are rather fixed in the molecular memory. Therefore, our findings prompt the re-thinking of the extrapolation of results from single-origin endothelial cell systems.
Overall design Whole transcriptome data of primary adult canine endothelial and vascular smooth muscle cells of 9 macrovessels, produced on Illumina NextSeq500 using a single-end 75 bp configuration
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Contributor(s) Spanjersberg TF, Oosterhoff LA, Kruitwagen HS, van den Dungen NM, Harakalova M, Mokry M, Spee B, van Steenbeek FG, Vernooij H
Citation(s) 37563195
Submission date Apr 03, 2021
Last update date Nov 21, 2023
Contact name Frank van Steenbeek
Phone 0302532848
Organization name Utrecht University
Street address Yalelaan 104
City Utrecht
State/province Select State/Province
ZIP/Postal code 3584 CM
Country Netherlands
Platforms (1)
GPL21400 Illumina NextSeq 500 (Canis lupus familiaris)
Samples (48)
GSM5225596 endothelial cells coronary artery dog A
GSM5225597 endothelial cells coronary artery dog C
GSM5225598 endothelial cells femoral artery dog A
BioProject PRJNA719562
SRA SRP313354

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Supplementary file Size Download File type/resource
GSE171437_MOK2599_readCounts_raw.txt.gz 603.5 Kb (ftp)(http) TXT
GSE171437_MOK2600_readCounts_raw.txt.gz 498.0 Kb (ftp)(http) TXT
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Processed data are available on Series record

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