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| Status |
Public on Mar 21, 2010 |
| Title |
Yeast background with high-concentration spikes |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
|
| Summary |
Microarray measurements are susceptible to error caused by cross-hybridization and bulk hybridization. To characterize the effect of these off-target interactions, we analyzed samples made from baseline (S. cerevisiae) alone or mixed with a high-concentration 1kb spike.
The spike is a 1 kb sequence amplified from the Schizosaccharomyces pombe gene SPAC27D7.10c, with an added oligo(dT)-T7 promoter. The baseline and spike samples were mixed at the double-stranded cDNA stage.
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| Overall design |
Three arrays: 100% baseline, 99% baseline + 1% spike, 90% baseline + 10% spike. There are no replicates.
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| Contributor(s) |
Eklund AC, Friis P, Wernersson R, Szallasi Z |
| Citation(s) |
19969549 |
| |
| Submission date |
Jul 07, 2009 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Aron C Eklund |
| Organization name |
Technical University of Denmark
|
| Department |
Center for Biological Sequence Analysis
|
| Street address |
Kemitorvet, Building 208
|
| City |
Lyngby |
| ZIP/Postal code |
2800 |
| Country |
Denmark |
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| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA117281 |
