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| Status |
Public on Feb 04, 2022 |
| Title |
Uhrf1 control the proliferation and the differentiation of satellite cells |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
DNA methylation is an essential form of epigenetic regulation responsible for cellular identity. In muscle stem cells, termed satellite cells, DNA methylation patterns are tightly regulated during differentiation. However, it is unclear how these DNA methylation patterns affect the function of satellite cells. We demonstrate that a key epigenetic regulator, ubiquitin like with PHD and RING finger domains 1 (Uhrf1), is activated in proliferating myogenic cells but not expressed in quiescent satellite cells or differentiated myogenic cells in mice. Ablation of Uhrf1 in mouse satellite cells impairs their proliferation and differentiation, leading to failed muscle regeneration. Uhrf1-deficient myogenic cells exhibited aberrant up-regulation of transcripts, including Sox9, with the reduction of DNA methylation level of their promoter and enhancer region. These findings show that Uhrf1 is a critical epigenetic regulator of proliferation and differentiation in satellite cells, by controlling cell type-specific gene expression via maintenance of DNA methylation.
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| |
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| Overall design |
Refer to individual Series
|
| |
|
| Citation(s) |
35243267 |
| |
| Submission date |
Mar 18, 2021 |
| Last update date |
Mar 06, 2022 |
| Contact name |
Hiroshi Sakai |
| E-mail(s) |
sakai.hiroshi.wh@ehime-u.ac.jp
|
| Phone |
+81-89-960-5925
|
| Organization name |
Ehime University
|
| Department |
Proteo-Science Center
|
| Lab |
Division of Integrative Pathophysiology
|
| Street address |
Shitsukawa
|
| City |
Toon |
| State/province |
Ehime |
| ZIP/Postal code |
791-0295 |
| Country |
Japan |
| |
|
| Platforms (2) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (13)
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| This SuperSeries is composed of the following SubSeries: |
| GSE169184 |
Uhrf1 control the proliferation and the differentiation of satellite cells [MBD-seq] |
| GSE169192 |
Uhrf1 control the proliferation and the differentiation of satellite cells [RNA-Seq] |
|
| Relations |
| BioProject |
PRJNA715510 |
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