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Series GSE168746 Query DataSets for GSE168746
Status Public on Dec 08, 2022
Title Bulk RNA-Seq datasets supporting fibroblast polarization drives skin regeneration versus fibrosis in adult reindeer
Organism Rangifer tarandus
Experiment type Expression profiling by high throughput sequencing
Summary In adult mammals, skin wound healing has evolved to favor rapid repair through formation of fibrotic scar. Consequently, skin wounds are dysfunctional and lead to chronic disfigurement and disability, yet the biologic mechanisms that drive fibrosis and prevent tissue regeneration remain unknown. Here, we report that reindeer (Rangifer tarandus) antler velvet exhibits regenerative wound healing, whereas identical full-thickness injury in dorsal back skin forms fibrotic scar. This regenerative capacity is retained even following ectopic transplantation of velvet to a scar-forming site, demonstrating that this latent regenerative capacity is innate to velvet cells and independent of local factors derived from the growing antler. Single cell RNA-sequencing of uninjured skin revealed a marked divergence in resting fibroblast transcriptional states and immunomodulatory function. Uninjured velvet fibroblast shared a striking resemblance with human fetal fibroblasts whereas uninjured back skin fibroblasts exhibited an overrepresentation of pro-inflammatory genes resembling adult human fibroblasts. Identical skin injury resulted in site-specific fibroblast polarization; back fibroblasts exacerbated the inflammatory response, whereas velvet fibroblasts adopted an immunosuppressive state and reverted back to a regeneration-competent ground state. Consequently, velvet wounds exhibited reduced immune infiltrate, accelerated adoption of anti-inflammatory immune states and expedited resolution of immune response. This study demonstrates reindeer as a novel mammalian model to study adult skin regeneration (velvet) and scar formation (back skin) within the same animal. Our study underscores the importance of fibroblast heterogeneity in shaping local immune cell functions that ultimately polarize wound healing outcomes. Purposeful, acute modulation of fibroblast-mediated immune signaling represents an important therapeutic avenue to mitigate scar and improve wound healing.
 
Overall design 34 samples were taken from 4 different animals, 2 wound sites, and 4 different time points. Expression values from Single-End bulk RNA-Seq.
 
Contributor(s) Sinha S, Sparks H, Robbins H, Gowing K, Jaffer A, Arora R, Raredon MS, Cao L, Swanson S, Jiang P, Hee O, Pope H, Labit E, Workentine M, Niklason L, Rosin N, Muench G, Stewart R, Matyas J, McCorkell R, Biernaskie J
Citation(s) 36493752
Submission date Mar 11, 2021
Last update date Mar 09, 2023
Contact name Jeff Biernaskie
E-mail(s) jabierna@ucalgary.ca
Phone 4032107306
Organization name University of Calgary
Department Comparative Biology and Experimental Medicine
Lab Biernaskie Lab
Street address 3330 Hospital Drive NW
City Calgary
State/province Alberta
ZIP/Postal code T2N4N1
Country Canada
 
Platforms (1)
GPL29839 Illumina HiSeq 3000 (Rangifer tarandus)
Samples (34)
GSM5165300 Reindeer_1A__Antler__day_0 62137
GSM5165301 Reindeer_1A__Back__day_0 62138
GSM5165302 Reindeer_1A__Antler__day_3 62139
This SubSeries is part of SuperSeries:
GSE168748 Skin regeneration is enabled in the absence of fibroblast inflammatory priming
Relations
BioProject PRJNA713917
SRA SRP310472

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SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE168746_genes.ec.tab.gz 1.1 Mb (ftp)(http) TAB
GSE168746_genes.tpm.tab.gz 1.1 Mb (ftp)(http) TAB
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Raw data are available in SRA
Processed data are available on Series record

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