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| Status |
Public on Mar 08, 2021 |
| Title |
Disulfiram inhbits M. tuberculosis growth by altering methionine pool and redox balance |
| Organism |
Mycobacterium tuberculosis H37Rv |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Methionine biosynthetic pathway, essential for the growth of Mycobacterium tuberculosis (Mtb) in the host, represents an attractive target for the development of novel anti-tuberculars. Here, we have biochemically characterized homoserine acetyl transferase (HSAT viz. MetA) of Mtb, which catalyses the first committed step of methionine and S-adenosylmethionine (SAM) biosynthesis. High-throughput screening of a 2300 compound library resulted in identification of thiram, an anti-fungal organosulfur compound, as the most potent MetA inhibitor. Further analysis of thiram analogs led to the identification of orally bioavailable disulfiram (DIS, an anti-alcoholism FDA approved drug) as a novel inhibitor of MetA. Both thiram and DIS restricted the growth of drug-sensitive and drug-resistant Mtb strains in a bactericidal manner. ThermoFlour assay demonstrated direct binding of DIS with MetA. Metabolomic and transcriptomic studies showed DIS mediated perturbation of methionine and redox homeostasis, respectively, in Mtb. In concordance, the effect of DIS on Mtb growth was partially rescued by supplementation with either L-methionine as well as N-acetyl cysteine, suggesting a multi-target killing mechanism. In Mtb-infected mice, DIS administration restricted bacterial growth, increased efficacy of isoniazid, ameliorated lung pathology, modulated lung immune cell landscape and protective immune response. Taken together, our results demonstrate that DIS can be repurposed for designing an effective anti-tubercular therapy.
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| Overall design |
To compare the transcriptomic profiles of RNA obtained from M. tuberculosis H37Rv exposed to either 1x or 10x MIC99 of disulfiram (TETD). Control M. tuberculosis were treated with DMSO.
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| Contributor(s) |
Chaudhary D, Singh R, Lum J, Lee B, Singhal A |
| Citation missing |
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| |
| Submission date |
Mar 08, 2021 |
| Last update date |
Mar 12, 2021 |
| Contact name |
Nicholas Ang |
| E-mail(s) |
angdzn@immunol.a-star.edu.sg
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| Organization name |
Singapore Immunology Network (SIgN)
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| Street address |
8a Biomedical Grove, Singapore 138648
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| City |
Singapore |
| ZIP/Postal code |
Singapore 138648 |
| Country |
Singapore |
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| Platforms (1) |
| GPL29823 |
Illumina HiSeq 4000 (Mycobacterium tuberculosis H37Rv) |
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| Samples (11)
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| Relations |
| BioProject |
PRJNA707489 |
| SRA |
SRP309742 |
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