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Series GSE168451 Query DataSets for GSE168451
Status Public on Mar 09, 2022
Title Tenogenic induction from induced pluripotent stem cells unveils the trajectory towards tenocyte differentiation.
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The musculoskeletal system is integrated by tendons that are characterized by expression of a functionally important transcription factor Scleraxis (Scx). To date, there has been no solid culture system for tenogenic differentiation. Here we developed the tenocyte induction method using induced pluripotent stem cells established from ScxGFP transgenic mice by monitoring fluorescence that reflects a series of dynamic differentiation process. Among several developmentally relevant factors, TGF-2 was the most potent inducer for differentiation of tenocytes expressing Tenomodulin, a mature differentiation marker. Single-cell RNA sequencing (scRNA-seq) revealed 11 distinct clusters including mature tenocyte population and tenogenic differentiation trajectory, which well recapitulates in vivo developmental process. Dataset of scRNA-seq and transcriptome of Scx deficient tendon enabled identification of Scx-related genes in which tendon-related genes are enriched. Collectively, this study leads to a new opportunity for tendon research and provides further insight into mechanistic understanding of the differentiation pathway to a tenogenic fate.
 
Overall design Single cell-based RNA-sequencing during tenogenic induction from iPSCs and profiling of Achilles tendon mRNA of 14-day old wild type (Wt) andScx -/- (KO) mice.
 
Contributor(s) Yoshimoto Y, Uezumi A, Ikemoto-Uezumi M, Yambe S, Sotomaru Y, Shukunami C, Ohkawa Y, Maehara K, Tanaka K
Citation(s) 35372337
Submission date Mar 08, 2021
Last update date Apr 07, 2022
Contact name Yasuyuki Ohkawa
E-mail(s) yohkawa@bioreg.kyushu-u.ac.jp
Organization name Medical Institute of Bioregulation
Lab Division of Transcriptomics
Street address 3-1-1 Maidashi
City Fukuoka
ZIP/Postal code 8128582
Country Japan
 
Platforms (1)
GPL18480 Illumina HiSeq 1500 (Mus musculus)
Samples (16)
GSM5140555 T0-1 single-cells
GSM5140556 T0-2 single-cells
GSM5140557 T0-3 single-cells
Relations
BioProject PRJNA707440
SRA SRP309712

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE168451_384_CellBarcode_list.txt.gz 1.3 Kb (ftp)(http) TXT
GSE168451_Achilles_tendon_bulk_RNA-seq_RawCount.csv.gz 309.1 Kb (ftp)(http) CSV
GSE168451_scRawCountAll.csv.gz 13.4 Mb (ftp)(http) CSV
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Raw data are available in SRA
Processed data are available on Series record

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