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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 01, 2022 |
| Title |
RNA sequencing microglia depletion model |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We developed an experimental model system in mice, in which microglia can be depleted selectively and temporarily from the prefrontal cortex (PFC) during a defined window of adolescence (6 weeks of age). This was achieved using a single, bilateral stereotaxic intra-PFC injection of clodronate disodium salt (CDS), which induces selective apoptosis of microglia. To ascertain the cellular selectivity of intra-PFC administration of CDS, we conducted next-generation RNA sequencing (RNAseq) of bulk medial PFC tissue from CDS- or phosphate buffered saline (PBS)-injected mice. PFC samples were extracted either during the peak of microglia depletion, that is, 5 days post injection (5 dpi) or in adulthood (12 weeks of age) after full microglia repopulation.
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| Overall design |
Next-generation RNA sequencing of mouse medial prefrontal cortex samples a) in adolescence (6 weeks of age), 5 days after intra-PFC PBS or CDS injection and b) in adulthood (12 weeks of age), 6 weeks after intra-PFC PBS or CDS injection.
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| Contributor(s) |
Schalbetter SM, von Arx AS, Cruz-Ochoa N, Dawson K, Ivanov A, Mueller FS, Lin H, Amport R, Mildenberger W, Mattei D, Beule D, Földy C, Greter M, Notter T, Meyer U |
| Citation(s) |
35235358 |
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| Submission date |
Mar 04, 2021 |
| Last update date |
Mar 30, 2022 |
| Contact name |
Sina-Maria Schalbetter |
| E-mail(s) |
sina.schalbetter@uzh.ch
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| Organization name |
Institute of Pharmacology and Toxicology, University of Zurich-Vetsuisse
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| Street address |
Winterthurerstrasse 260
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| City |
Zurich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA706711 |
| SRA |
SRP309331 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE168268_Gene_expression_changes_in_CDS_mice_versus_PBS_mice_at_12_weeks_of_age.xlsx |
896.2 Kb |
(ftp)(http) |
XLSX |
| GSE168268_Gene_expression_changes_in_CDS_mice_versus_PBS_mice_at_5dpi.xlsx |
1011.5 Kb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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