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Series GSE168255 Query DataSets for GSE168255
Status Public on Mar 08, 2022
Title The RNA Helicase DHX16 Provides Specific Viral RNA for RIG-I to Trigger Innate Antiviral Immunity
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Type-I interferon (IFN-I) is essential to establish antiviral innate immunity. Unanchored (or free) polyubiquitin (poly-Ub) has been shown to regulate IFN-I responses, however few unanchored poly-Ub interactors are known. To identify factors regulated by unanchored poly-Ub in a physiological setting, we developed an approach to isolate unanchored poly-Ub from lung tissue and identified the DEAH-box RNA helicase DHX16 as a factor that enhances IFN-I production. Silencing of DHX16 in cells and in vivo diminished IFN-I responses against influenza virus. DHX16-dependent IFN-I production requires RIG-I and unanchored K48-poly-Ub synthesized by the E3 Ub ligase TRIM6. DHX16 recognizes a signal in influenza segments that undergo splicing and requires its intact RNA helicase motif for direct, high-affinity interactions with specific viral RNAs. Our study establishes DHX16 as a potentiator of RIG-I-mediated IFN-I production and recognizes a mechanism for activation of antiviral immunity requiring unanchored poly-Ub and the viral RNA recognition activity of DHX16
Overall design Next Generation Sequencing of human A549 lung cells treated with either non-targeting control or DHX16 targeting siRNA and either mock or influenza A virus infected (MOI=1) for 24 hours.
Contributor(s) Hage A, Bharaj P, van Tol S, Giraldo MI, Aguilera-Aguirre L, Xie X, Widen SG, Moulton HM, Johnson JR, Krogan NJ, García-Sastre A, Shi P, Freiberg AN, Rajsbaum R
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Submission date Mar 04, 2021
Last update date Mar 08, 2022
Contact name Adam Hage
Organization name University of Texas Medical Branch at Galveston
Department Microbiology and Immunology
Lab Rajsbaum Lab
Street address 301 University Blvd
City Galveston
State/province Texas
ZIP/Postal code 77555
Country USA
Platforms (1)
GPL18460 Illumina HiSeq 1500 (Homo sapiens)
Samples (8)
GSM5135054 siNTC-1_Mock
GSM5135055 siNTC-2_Mock
GSM5135056 siDHX16-1_Mock
BioProject PRJNA706691
SRA SRP309315

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Supplementary file Size Download File type/resource
GSE168255_all.counts.tsv.gz 363.0 Kb (ftp)(http) TSV
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