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Series GSE16804 Query DataSets for GSE16804
Status Public on Oct 28, 2010
Title Analysis of transcriptome in ectopic versus orthotopic thyroid tissue.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Congenital hypothyroidism from thyroid dysgenesis (CHTD) is a sporadic disease characterized by defects in the differentiation, migration or growth of thyroid tissue. Of these defects, incomplete migration resulting in ectopic thyroid tissue is the most common (up to 80%). We obtained flashfrozen samples of ectopic thyroid tissue removed from 3 girls aged 8, 10 and 15 yr, because it caused local symptoms. For comparison, we used orthotopic thyroid tissue from a Caucasian female, age 68 with gall bladder cancer. Analysis of transcriptome revealed up to 1011 genes more than twofold induced or repressed.
Overall design Microarray hybridization was performed on three independent pairs of ectopic thyroids (one pair performed in duplicate) compared to control thyroid tissue. After amplification and labelling, sample pairs were hybridized onto Human Exonic Evidence Based Oligonucleotide HEEBO slides (Stanford Functional Genomics Facility, CA). The oligonucleotide set consists of 44544 70-mer probes that were designed using a transcriptome-based annotation of exonic structure for genomic loci. Hybridizations were replicated with dye swap.
Contributor(s) Abu-Khudir R, Paquette J, Lefort A, Libert F, Chanoine J, Vassart G, Van Vliet G, Deladoëy J
Citation(s) 20976176
Submission date Jun 25, 2009
Last update date Aug 28, 2014
Contact name Frederick Libert
Organization name ULB
Department IRIBHM
Lab C2-140
Street address 808 route de Lennik
City Brussels
ZIP/Postal code B-1070
Country Belgium
Platforms (1)
GPL6254 Phalanx Human OneArray
Samples (8)
GSM422030 HC22 versus normal Thyroid, biological replica 1_1
GSM422031 HC22 versus normal Thyroid, biological replica 1_2
GSM422032 HC23 versus normal Thyroid, biological replica 2_1
BioProject PRJNA117519

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE16804_RAW.tar 26.5 Mb (http)(custom) TAR (of GPR)
Processed data included within Sample table

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