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| Status |
Public on Oct 19, 2021 |
| Title |
Intranasal administration of OM-85, a bacterial lysate, alters lung transcriptional profiles and protects against experimental asthma |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Background: Microbial interventions against allergic asthma have robust epidemiologic underpinnings and the potential to recalibrate disease-inducing immune responses. Oral administration of OM-85, a standardized lysate of human airways bacteria, is widely used empirically to prevent respiratory infections, and a clinical trial is testing its ability to prevent asthma in at-risk children. On the other hand, we previously showed that intra-nasal administration of products from microbe-rich farm environments abrogate experimental allergic asthma.
Objectives: To investigate whether direct administration of OM-85 to the airway compartment protects against experimental allergic asthma, and to identify protective cellular and molecular mechanisms activated through this natural route.
Methods: BALB/cJ mice (7-8 weeks old) sensitized and challenged with Ovalbumin received OM-85 intra-nasally, and cardinal cellular and molecular asthma phenotypes were measured. Murine lung gene expression was profiled by RNA-sequencing.
Results: Airway administration of OM-85 suppressed allergic asthma and altered the transcriptome profile in unfractionated lung tissue.
Conclusion We provide the first demonstration that administration of a standardized bacterial lysate to the airway compartment protects from experimental allergic asthma by engaging multiple immune pathways.
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| Overall design |
Transcriptome profiling in unfractionated lung tissue from mice treated intranasally with bacterial lysate (OM-85)
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| Contributor(s) |
Vercelli D, Pivniouk V, DeVries A |
| Citation(s) |
34560105 |
| |
| Submission date |
Feb 27, 2021 |
| Last update date |
Oct 19, 2021 |
| Contact name |
Donata Vercelli |
| Organization name |
University of Arizona
|
| Street address |
1657 E. Helen St.
|
| City |
Tucson |
| State/province |
Arizona |
| ZIP/Postal code |
85721 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (27)
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| Relations |
| BioProject |
PRJNA705359 |
| SRA |
SRP308486 |
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